Leukemia-mutated proteins PHF6 and PHIP form a chromatin complex that represses myeloid leukemia stemness.

Myeloid leukemias are heterogeneous cancers with a diverse mutational landscape. Though many mutated genes fall within common protein complexes, some lack known functional partners and have unclear roles. PHF6 is a poorly-understood chromatin-binding protein with recurrent mutations that confer an unfavorable prognosis in acute and chronic myeloid leukemias.

Protocol for immunodot blot detection of UVB photoproducts in extracellular DNA.

UV radiation induces the formation of adducts in genomic DNA within cells that are later found to be present in cell-free fractions associated with extracellular vesicles (EVs) outside of cells. Here, we present a protocol for isolating UV photoproducts in extracellular DNA released from UVB-irradiated cells via differential centrifugation. We then detail steps for monitoring the DNA adducts using DNA immunoblotting.

Comparative Role of Chain Scission and Solvation in the Biodegradation of Polylactic Acid (PLA).

The molecular mechanism behind the process of biodegradation and consequently the loss in mechanical properties of polylactic acid (PLA) requires detailed understanding for the successful designing of various technological devices. In this study, we examine the role of free water and chain scission in this degradation process and quantify the mechanical properties of pristine and nanoparticle-reinforced PLA as it degrades over time. The in situ mechanical response of the degrading polymer is determined experimentally using nano-dynamic mechanical analysis (nanoDMA).