Publications by authors named "Alec Callisto"
Article Synopsis
- High-resolution cellular signal encoding is essential for understanding complex biological phenomena, utilizing DNA-based biosignal encoders that respond to specific signals to modify genomic DNA.
- The system presented employs terminal deoxynucleotidyl transferase (TdT) to incorporate nucleotides into DNA in response to specific biosignals, using CRISPR-Cas9 to create necessary substrate ends for the enzyme's action.
- This methodology successfully encodes various biosignal concentrations into HEK-293T cells, achieving a 91% accuracy in embedding the message "HELLO WORLD," showcasing a reliable way to store biosignal information in mammalian cell genomes.
Employing DNA as a high-density data storage medium has paved the way for next-generation digital storage and biosensing technologies. However, the multipart architecture of current DNA-based recording techniques renders them inherently slow and incapable of recording fluctuating signals with subhour frequencies. To address this limitation, we developed a simplified system employing a single enzyme, terminal deoxynucleotidyl transferase (TdT), to transduce environmental signals into DNA.
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