Construction of a rat spinal cord atlas of axon morphometry.

Atlases of the central nervous system are essential for understanding the pathophysiology of neurological diseases, which remains one of the greatest challenges in neuroscience research today. These atlases provide insight into the underlying white matter microstructure and have been created from a variety of animal models, including rats. Although existing atlases of the rat spinal cord provide some details of axon microstructure, there is currently no histological dataset that quantifies axon morphometry exhaustively in the entire spinal cord.

AxonDeepSeg: automatic axon and myelin segmentation from microscopy data using convolutional neural networks.

Segmentation of axon and myelin from microscopy images of the nervous system provides useful quantitative information about the tissue microstructure, such as axon density and myelin thickness. This could be used for instance to document cell morphometry across species, or to validate novel non-invasive quantitative magnetic resonance imaging techniques. Most currently-available segmentation algorithms are based on standard image processing and usually require multiple processing steps and/or parameter tuning by the user to adapt to different modalities.

AxonSeg: Open Source Software for Axon and Myelin Segmentation and Morphometric Analysis.

Segmenting axon and myelin from microscopic images is relevant for studying the peripheral and central nervous system and for validating new MRI techniques that aim at quantifying tissue microstructure. While several software packages have been proposed, their interface is sometimes limited and/or they are designed to work with a specific modality (e.g.

Specific Adsorption via Peptide Tags: Oriented Grafting and Release of Growth Factors for Tissue Engineering.

Numerous strategies have been proposed to decorate biomaterials with growth factors (GFs) for tissue engineering applications; their practicability as clinical tools, however, remains uncertain. We previously presented two complementary amphipathic peptides, namely, E5 and K5, which could be utilized as tags to direct GF capture onto organic materials via E5/K5 coiled-coil interactions. We here investigated their potential as mediators of GF physical adsorption.