Biotransformation of cladribine by a magnetic immobilizated biocatalyst of Lactobacillus animalis.

A stable biocatalyst with magnetic properties based on immobilized Lactobacillus animalis ATCC 35,046 to obtain 2-chloroadenine-2'-deoxyriboside, known as cladribine, is reported for the first time. This nucleoside analogue is an antitumor agent used in the treatment of a wide variety of types of leukemia. In this study, an eco-compatible and alternative bioprocess to obtain cladribine was developed.

Saccharification of citrus wastes by immobilized polygalacturonase in an improved alginate matrix.

Enzyme immobilization using hydrogels is a low-cost and effective system for the degradation of bulk pectin derived from orange industry residues. Polygalacturonases obtained from four different bacterial strains of genus were immobilized in alginate gel and assayed for pectin hydrolysis. The enzyme from ATCC 10897 proved to be superior and more stable within the alginate matrix.