Liquid-based cytology in DNA-based molecular research: viability and potential application.

Objective: To evaluate the effectiveness of UCM and PreservCyt liquid media in molecular analysis using exfoliated oral cells and analyze Tp53 codon 72 extracted from these cells.

Study Design: Exfoliated oral cells were placed in UCM and PreservCyt preservation media. DNA was isolated by proteinase K/phenol-chloroform protocol.

KIT activation in uterine cervix adenosquamous carcinomas by KIT/SCF autocrine/paracrine stimulation loops.

Objectives: Uterine adenosquamous carcinoma (ASC) is an uncommon, yet, one of the most aggressive cervical cancer subtype. The successful treatment of some tumors, such as gastrointestinal stromal tumors (GISTs), by anti-KIT inhibitors fosters the study of this receptor tyrosine kinase in other malignancies. In the present study, we intended to molecularly characterize KIT in ASC.