Pretreatment of Vine Shoot Biomass by Choline Chloride-Based Deep Eutectic Solvents to Promote Biomass Fractionation and Enhance Sugar Production.

Vine shoots hold promise as a biomass source for fermentable sugars with efficient fractionation and conversion processes. The study explores vine shoots as a biomass source for fermentable sugars through pretreatment with two deep eutectic solvents mixtures: choline chloride:lactic acid 1:5 (ChCl:LA) and choline chloride:ethylene glycol 1:2 (ChCl:EG). Pretreatment conditions, such as temperature/time, solid/liquid ratio, and biomass particle size, were studied.