Background: We undertook this study to present biochemical and morphological characterization of serotonergic cells during fetal heart development.
Methods: Wistar rats (10, 12, 16 and 20 days of gestation) were used. After obtaining the fetuses by Cesarean section, the hearts were removed and fixed for immunohistochemical assay of tryptophan-5-hydroxylase (Tph), in addition to Western blot for enzyme.