Effect of a trivalent vaccine against Staphylococcus aureus mastitis lymphocyte subpopulations, antibody production, and neutrophil phagocytosis.

The effect of a novel bovine mastitis trivalent vaccine, containing Staphylococcus aureus capsular polysaccharide type 5 (T5), 8 (T8), and 336 (T336), on lymphocyte subpopulations, antibody production, and neutrophil phagocytosis was evaluated. Twenty pregnant heifers were immunized with either the trivalent alone, trivalent emulsified in Freund's incomplete adjuvant (FICA), trivalent in aluminum hydroxide, or adjuvant only (FICA). Immunization was done 30 d before the expected calving date followed by 2 boosts in a 2-week interval.

Differences in Absorbance (OD) of Various Strains of Staphylococcus aureus as Determined by Direct Microscopic Counts.

A modification of the Breed direct microscopic method for counting Staphylococcus aureus and its use in preparing standard curves with the spectrophotometer is described. Eight strains of S. aureus were grown on various media.