The karyotypes and chromosomal characteristics of three Eigenmann et Kennedy, 1903 species were examined using conventional and molecular protocols. These species had invariably a diploid chromosome number 2n = 50. (Block, 1794) and (Cuvier, 1819) had the karyotype composed of 16 metacentric (m) + 28 submetacentric (sm) + 6 subtelocentric (st) chromosomes while (Schomburgk, 1841) had the karyotype composed of 14m+30sm+6st elements.
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