Publications by authors named "Albane Gaubert"
Anti-silencing function 1 (ASF1) is a conserved H3-H4 histone chaperone involved in histone dynamics during replication, transcription, and DNA repair. Overexpressed in proliferating tissues including many tumors, ASF1 has emerged as a promising therapeutic target. Here, we combine structural, computational, and biochemical approaches to design peptides that inhibit the ASF1-histone interaction.
View Article and Find Full Text PDFMCM2 is a subunit of the replicative helicase machinery shown to interact with histones H3 and H4 during the replication process through its N-terminal domain. During replication, this interaction has been proposed to assist disassembly and assembly of nucleosomes on DNA. However, how this interaction participates in crosstalk with histone chaperones at the replication fork remains to be elucidated.
View Article and Find Full Text PDFSurprising complexity of the Asf1 histone chaperone-Rad53 kinase interaction.
Proc Natl Acad Sci U S A
February 2012
Article Synopsis
- The histone chaperone Asf1 and the checkpoint kinase Rad53 form a complex in yeast cells without genotoxic stress, involving three key interaction sites.
- One binding site includes Asf1's H3-binding surface interacting with Rad53, while another involves Rad53's FHA1 domain binding to the phosphorylated Asf1.
- The stability of the Asf1-Rad53 complex affects the DNA damage response, with a specific rad53 mutation leading to increased viability of certain mutant cells during genotoxic stress.