Exploring the Kinetics and Thermodynamics of a Novel Histidine Ammonia-Lyase from .

Histidine ammonia-lyase (HAL) plays a pivotal role in the non-oxidative deamination of L-histidine to produce -urocanic, a crucial process in amino acid metabolism. This study examines the cloning, purification, and biochemical characterization of a novel HAL from (HAL) and eight active site mutants to assess their effects on substrate binding, catalysis, thermostability, and secondary structure. The HAL enzyme was successfully overexpressed and purified to homogeneity.