[Insulin production of isolated fresh and cryopreserved islands of Langerhans of rats].

The effect of glucose and Domperidon (R 33812, pharmaceutical factory Gedeon Richter, Budapest) on the insulin secretion were investigated in the isolated islets of Langerhans of rats. The insulin secretion was increased by glucose and also by Domperidon, however, in a smaller size. The insulin secretion of cryopreserved islets--stored in liquid nitrogen of -196 degrees C during 2-6 months--was equal with the secretion of fresh isolated islets.

[Transplantation of islands of Langerhans: inclusion of living cells into a polymer forming a net-like structure].

A two-component polyacrylic amide gel forming a net-like structure was built up on pancreatic cells. In such a way the transplanted cells could be protected from the damage by immune protein with big molecule. The method was tested in yeast cells before, human erythrocytes, and wether erythrocytes.

[Structure of adrenal glands removed for hyperandrogenism and their hormone production in tissue culture].

Authors studied the surgically removed adrenals of a 16 year old girl with hyperandrogenism in tissue culture. The majority of cells in the initial culture was similar to the cells of the normal human adrenal. In the 6 weeks tissue culture the morphology of the cells has shown an alteration.

First clinical experience with a new neuromuscular blocker pipecurium bromide.

The clinical trial of the new non-depolarizing muscle relaxant 2 beta,16 beta-bis-(4'-dimethyl-1'-piperazino)-3 alpha,17 beta diacetoxy-5 alpha-androstane dibromide (pipecurium bromide, RGH-1106, Arduan) was performed on 80 patients who had to undergo medium and major size intraabdominal operation on the basis of chronic surgical indication. Four types of general anaesthetic methods were applied, neuroleptanalgesia, halothane, halothane-fentanyl combination and hydroxydione. The clinical applicability of pipecurium bromide was tried to establish the dose needed for complete muscle relaxation initially and repeatedly and the effect of antagonist drugs on the muscle relaxation.

Respiration paralysing and circulatory effects of a new non-depolarizing relaxant, pipecurium bromide, in anaesthetized dogs.

The effect of a new sterane-structure, non-depolarizing muscle relaxant 2 beta,16 beta-bis(4'-dimethyl-1'-piperazino)-3 alpha,17 beta-diacetoxy-5 alpha-androstane dibromide (pipecurium bromide, RGH-1106, Arduan) was studied in 42 mongrel dogs under general anaesthesia (ether, halothane, methoxyflurane, thiobutobarbital, hydroxydione and dehydrobenzperidol-fentanyl (NLA II). Onset and duration of action and the presence of circulatory side effects were examined. It was found that the mean onset of respiration paralysing action was 156.

[Rapid determination of antibiotics resistance in clinico-surgical practice on the basis of radioactive carbon dioxide measurements].

The radiometric method is founded on the measurement of the 14CO2 which has gone free. This method was worked out of our team. It is a prompt sensitive and quantitative procedure for the control of antibiotic effect on bacteria.

[Changes in the Na+, K+, and Ca++ concentrations in the urine in the course of surgical interventions].

The tests were carried out on 27 surgical patients. Diuresis and the Na, K and Ca concentrations in the urine were measured before, during and after operation. Due to operational stress and to the drugs used for general anaesthesia diuresis decreases progressively during operation and compared to the conscious state the amount of cations excreted during operations is significantly lower.

[Changes in kidney function in abdominoperineal rectum excision under halothane anesthesia].

In 17 cases of abdomino-perineal rectum extirpation performed under combined intratracheal halothane anaesthesia, the volume of urine and the osmolality of serum and urine were determined. Though urinary volume decreased significantly, its osmolality did not rise. According to the operational stress this type of operation can be divided into an: 1) intra-abdominal and a 2) perineal phase.

The effect of surgery on carbohydrate metabolism. II. Insulin response during surgery.

Serum-immunoreactive insulin response on glucose load was observed during the operation of 13 patients. The tests were performed on the same patients also 5 days prior to operation. The patients were divided into two groups according to the performed operation which was either intra- or extraperitoneal.

[Ultrastructure and hormone secretion of a tissue culture from an adrenal gland tumor simulating Cushing's syndrome].

Tissue cultures of a surgically removed adrenocortical tumour causing Cushing's syndrome, and tissue cultures from the attached, tumour-free adrenal were studied. There were two cell types characteristic of tumour tissue. The cell type occurring most frequently had pronounced hypertrophied agranular endoplasmic reticulum.

Changes in the fine structure and function of a hormone-secreting adrenocortical tumour investigated in tissue culture.

Tissue cultures of a surgically removed adrenocortical tumour causing Cushing's syndrome, and tissue cultures from the attached, tumour-free adrenal were studied. There were two cell types characteristic of tumour tissue. The cell type occurring most frequently had pronounced hypertrophied agranular endoplasmic reticulum.

Effect of surgery on carbohydrate metabolism.

The effect of surgery on carbohydrate metabolism has been studied in 20 patients. The blood sugar and serum immunoreactive insulin (IRI) levels were determined during various operations. The blood sugar level rose progressively with the duration of the intervention.

Changes in blood coagulation due to pancreas transplantation in dogs.

Blood coagulation was studied in the course of 11 pancreatectomies and 7 pancreas transplantations. Comparison of the postoperative changes in the fibrinogen level suggests the important role of the normal pancreas in fibrinogen generation. Investigation of the indices of the coagulation and fibrinolytic system revealed in the post-transplantation period a shift in the activity of the coagulation indices towards hypercoagulability.