Phospholipase D (PLD) has been implicated in mitotic regulation and has been shown to be defective in cells following replicative senescence. We examined the source of changes in PLD activity in senescent human umbilical vein endothelial cells and in human diploid fibroblasts. Using fractionated cell components we found that the cytosolic components were necessary for maximum PLD activation.
View Article and Find Full Text PDFBackground: Anecdotal and experimental evidence suggest that a sitting position with maximum knee extension, hip adduction, and forward lean (hamstring stretch position) may produce better reversal of the lumbar lordosis than a traditional sitting position.
Methods: In a randomized trial during initiation of epidural labor analgesia, we compared the traditional versus hamstring stretch positions. The primary outcome was the number of needle-bone contacts.