Generating realistic data through modeling and parametric probability for the numerical evaluation of data processing algorithms in two-dimensional chromatography.

Background: Comprehensive two-dimensional chromatography generates complex data sets, and numerous baseline correction and noise removal algorithms have been proposed in the past decade to address this challenge. However, evaluating their performance objectively is currently not possible due to a lack of objective data.

Result: To tackle this issue, we introduce a versatile platform that models and reconstructs single-trace two-dimensional chromatography data, preserving peak parameters.

Semi-continuous monitoring of HMWPAH in microalgae cultures by PT-SPE/HPLC/FD-UV: Estimation of the degradation constant.

A degradation study has been performed with Selenastrum capricornutum incubated with benzo[a]anthracene and benzo[a]pyrene at 50, 100 and 266 ng mL in liquid cultures. After incubation, these high molecular weight polycyclic aromatic hydrocarbons (HMW PAH) were extracted from both, the medium and biomass in a single step, and then quantified by a sensitive and validated analytical methodology based on pipette-tip SPE and HPLC with fluorescence and UV detection (PT-SPE/HPLC/FD-UV). The methodology presented good linearity r > 0.

Supercritical fluid chromatography as a rapid single-step method for the determination of mineral oil saturated and aromatic hydrocarbons in purified mineral oils for food and cosmetics applications.

Mineral oil hydrocarbons are used in the consumer goods sector for the elaboration of a wide range of foods and cosmetics. Traditional methods for determining their levels and composition are time consuming and laborious, besides requiring complex instrumentation. Here a simple and fast method was developed that uses columns packed with silver-modified silica in supercritical fluid chromatography with flame ionization and UV detection (SFC-FID/UV) for the determination of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) in purified mineral oil samples.

Comprehensive off-line silver phase liquid chromatography × gas chromatography with flame ionization and vacuum ultraviolet detection for the detailed characterization of mineral oil aromatic hydrocarbons.

Highly purified mineral oils used for the elaboration of pharmaceutical, food and cosmetic products can contain residual mineral oil aromatic hydrocarbons (MOAH). Quantification of the MOAH level as well as detailed characterization of the aromatic species present is important for safety evaluations and for optimization of the purification process. Two comprehensive off-line silver phase liquid chromatography × gas chromatography (AgLC × GC) methods, one with flame ionization detection (FID) and another with vacuum ultraviolet detection (VUV), were developed for MOAH analysis.

Direct analysis of aromatic hydrocarbons in purified mineral oils for foods and cosmetics applications using gas chromatography with vacuum ultraviolet detection.

Highly purified mineral oils are used in several pharmaceutical, foods and cosmetics applications. A fast and simple method was developed for the analysis of the total level of residual mineral oil aromatic hydrocarbons (MOAH) in these oils and in the intermediate oils that were sampled during the purification process. The method is based on gas chromatography with vacuum ultraviolet detection (GC-VUV) and relies on the spectral differences between the aliphatic and aromatic compounds in the sample.

On-line SPE chromatography with spectrophotometric diode array detection as a simple and advantageous choice for the selective trace analysis of benzo(a)anthracene degradation products from microalgae.

In this work, a methodology based on on-line solid phase extraction (SPE) chromatography with spectrophotometric diode array detection was optimized and validated for the trace analysis of benzo(a)anthracene dihydrodiol degradation products from microalgae cultures 5,6-dihydrodiol, 8,9-dihydrodiol and 10,11-dihydrodiol. The two on-line methods for the constituents of the culture, an SPE/on-line SPE chromatographic method for liquid medium and a matrix solid phase dispersion (MSPD)/on-line SPE chromatographic method for biomass presented good linearity in the ranges of 0.5-47ngmL and 2-80ngmg of samples, respectively, with correlation coefficients r>0.