Publications by authors named "Alan Kemp"

Egyptian rousette bats (ERBs) are implicated as reservoir hosts for Marburg virus (MARV), but natural mechanisms involved in maintenance of MARV in ERB populations remain undefined. A number of hematophagous ectoparasites, including fleas, parasitize bats. Subcutaneous (SC) inoculation of ERBs with MARV consistently results in viremia, suggesting that infectious MARV could be ingested by blood-sucking ectoparasites during feeding.

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Article Synopsis
  • Rift Valley fever virus (RVFV) is a significant mosquito-borne virus affecting livestock and humans, particularly in north-eastern KwaZulu-Natal, South Africa, where the mosquito vectors are not well understood.
  • The study collected blood-fed mosquitoes over several years (2019-2023) to identify their blood meal sources and understand the relationships between mosquitoes and their hosts, using genetic sequencing techniques.
  • Results indicated that 88.8% of the blood meals came from mammals, with cattle and nyala being the most common hosts, while all tested mosquitoes were found negative for RVFV, suggesting low immediate risk in the area.
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Article Synopsis
  • Rift Valley fever virus (RVFV) is a mosquito-borne disease affecting humans and animals in Africa and some nearby regions, with outbreaks linked to heavy rainfall that increases mosquito populations.
  • Recent studies in KwaZulu-Natal, South Africa found RVFV in a specific mosquito species, suggesting it may play a role in spreading the virus in this endemic area.
  • The identified strain of the virus is closely related to historical strains from outbreaks over 60 years ago, underscoring the need for further research on RVFV's ecology and its mosquito vectors in southern Africa.
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Rift Valley fever virus (RVFV) activity in Southern Africa tends to occur during periods of sustained elevated rainfall, cooler than normal conditions, and abundant vegetation cover creating ideal conditions for the increase and propagation of populations of RVFV mosquito vectors. These climatic and ecological conditions are modulated by large-scale tropical-wide -Southern Oscillation (ENSO) phenomena. The aim of this 5-year study was to investigate climatic conditions during Rift Valley fever "post-epizootic" period in Free State province of the Republic of South Africa, which historically experienced the largest RVF outbreaks in this country.

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This study aimed to determine the vector competence of bat-associated nycteribiid flies () for Marburg virus (MARV) in the Egyptian Rousette Bat (ERB), . In flies fed on subcutaneously infected ERBs and tested from 3 to 43 days post infection (dpi), MARV was detected only in those that took blood during the peak of viremia, 5-7 dpi. Seroconversion did not occur in control bats in contact with MARV-infected bats infested with bat flies up to 43 days post exposure.

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We conducted a survey for group-specific indirect immunofluorescence antibody to mammarenaviruses by using Lassa fever and Mopeia virus antigens on serum specimens of 5,363 rodents of 33 species collected in South Africa and Zimbabwe during 1964-1994. Rodents were collected for unrelated purposes or for this study and stored at -70°C. We found antibody to be widely distributed in the 2 countries; antibody was detected in serum specimens of 1.

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Article Synopsis
  • * A study conducted in central South Africa from 2017 to 2018 revealed a high IgG seroprevalence of 74.2% in cattle and a significantly lower 3.9% in farm and wildlife workers, indicating widespread exposure and varying risk factors among different age groups and occupations.
  • * Findings highlight that actual CCHF infections are likely underreported, with many undiagnosed cases, stressing the urgent need for improved public health strategies, education, and preventive
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Outbreaks of Rift Valley fever have devastating impacts on ruminants, humans, as well as on regional and national economies. Although numerous studies on the impact and outbreak of Rift Valley fever exist, relatively little is known about the role of environmental factors, especially soil, on the aestivation of the virus. This study thus selected 22 sites for study in central South Africa, known to be the recurrent epicenter of widespread Rift Valley fever outbreaks in Southern Africa.

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Rift Valley fever (RVF) is a mosquito-borne viral zoonosis showing complex epidemiological patterns that are poorly understood in South Africa. Large outbreaks occur in the central interior at long, irregular intervals, most recently in 2010-2011; however, the level of herd immunity of ruminant livestock, a key determinant of outbreaks, is unknown. During 2015-2016 a cross-sectional study on 234 randomly-selected farms investigated the prevalence, patterns of, and factors associated with, antibodies to RVF virus (RVFV) in livestock in an area heavily affected by that outbreak.

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Many lakes undergo anthropogenically driven eutrophication and pollution leading to decreased water and sediment quality. These effects can enhance seasonally changing lake redox conditions that may concentrate potentially toxic elements. Here we report the results of a multi-method geochemical and sediment microfabric analysis applied to reconstruct the history of cultural eutrophication and pollution of the North and South Basins of Windermere, UK.

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A high diversity of corona- and paramyxoviruses have been detected in different bat species at study sites worldwide, including Africa, however no biosurveillance studies from Rwanda have been reported. In this study, samples from bats collected from caves in Ruhengeri, Rwanda, were tested for the presence of corona- and paramyxoviral RNA using reverse transcription PCR assays. Positive results were further characterized by DNA sequencing and phylogenetic analysis.

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We report an external cavity diamond Raman laser operating at 2.52 μm, pumped by a 1.89 μm Tm:LiYF4 (YLF) laser.

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We detected a high seroprevalence of Marburg virus (MARV) antibodies in fruit bats in South Africa; 19.1% of recaptured bats seroconverted. The MARV RNA isolated closely resembled the 1975 Ozolin strain.

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In 2018, the family Arenaviridae was expanded by inclusion of 1 new genus and 5 novel species. At the same time, the recently established order Bunyavirales was expanded by 3 species. This article presents the updated taxonomy of the family Arenaviridae and the order Bunyavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV) and summarizes additional taxonomic proposals that may affect the order in the near future.

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We report a broadly wavelength-tunable femtosecond diode-pumped Ti:sapphire laser, passively mode-locked using both semiconductor saturable absorber mirror (SESAM) and Kerr-lens mode-locking (KLM) techniques. Using two pump laser diodes (operating at 450 nm), an average output power as high as 433 mW is generated during mode-locking with the SESAM. A tunability range of 37 nm (788-825 nm) was achieved with the shortest pulse duration of 62 fs at 812 nm.

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Rift Valley fever (RVF) is endemic in Africa and parts of the Middle East. It is an emerging zoonotic disease threat to veterinary and public health. Outbreaks of the disease have severe socio-economic impacts.

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The longest wavelength (~1.4 µm) emitted by a diamond Raman laser pumped by a semiconductor disk laser (SDL) is reported. The output power of the intracavity-pumped Raman laser reached a maximum of 2.

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Background: In August 2014, the National Institute for Communicable Diseases (NICD) in South Africa established a modular high-biosafety field Ebola diagnostic laboratory (SA FEDL) near Freetown, Sierra Leone in response to the rapidly increasing number of Ebola virus disease (EVD) cases.

Methods And Findings: The SA FEDL operated in the Western Area of Sierra Leone, which remained a "hotspot" of the EVD epidemic for months. The FEDL was the only diagnostic capacity available to respond to the overwhelming demand for rapid EVD laboratory diagnosis for several weeks in the initial stages of the EVD crisis in the capital of Sierra Leone.

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The Bunyaviridae family comprises viruses causing diseases of public and veterinary health importance, including viral haemorrhagic and arboviral fevers. We report the isolation, identification and genome characterization of a novel orthobunyavirus, named Wolkberg virus (WBV), from wingless bat fly ectoparasites (Eucampsipoda africana) of Egyptian fruit bats (Rousettus aegyptiacus) in South Africa. Complete genome sequence data of WBV suggests it is most closely related to two bat viruses (Mojuí dos Campos and Kaeng Khoi viruses) and an arbovirus (Nyando virus) previously shown to infect humans.

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Background: In a mosquito sterile insect technique programme the ideal scenario is to release male mosquitoes only. However, because there are currently no sex separation strategies which guarantee total female elimination, this study investigated the effect of irradiation on physiological and reproductive fitness of females of an Anopheles arabiensis genetic sexing strain.

Methods: Female pupae were irradiated at 70 Gy and the effects of irradiation on adult emergence, longevity, blood-feeding capability, mating ability, fecundity and fertility were assessed.

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Background: In Northern Botswana, rural communities, livestock, wildlife and large numbers of mosquitoes cohabitate around permanent waters of the Okavango Delta. As in other regions of sub-Saharan Africa, Rift Valley Fever (RVF) virus is known to circulate in that area among wild and domestic animals. However, the diversity and composition of potential RVF mosquito vectors in that area are unknown as well as the climatic and ecological drivers susceptible to affect their population dynamics.

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We report on the isolation of a novel fusogenic orthoreovirus from bat flies (Eucampsipoda africana) associated with Egyptian fruit bats (Rousettus aegyptiacus) collected in South Africa. Complete sequences of the ten dsRNA genome segments of the virus, tentatively named Mahlapitsi virus (MAHLV), were determined. Phylogenetic analysis places this virus into a distinct clade with Baboon orthoreovirus, Bush viper reovirus and the bat-associated Broome virus.

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Background: Anopheles arabiensis Patton is primarily responsible for malaria transmission in South Africa after successful suppression of other major vector species using indoor spraying of residual insecticides. Control of An. arabiensis using current insecticide based approaches is proving difficult owing to the development of insecticide resistance, and variable feeding and resting behaviours.

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Colonized Egyptian fruit bats (Rousettus aegyptiacus), originating in South Africa, were inoculated subcutaneously with Ebola virus (EBOV). No overt signs of morbidity, mortality, or gross lesions were noted. Bats seroconverted by Day 10-16 post inoculation (p.

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