Unamplified, Long-Read Metagenomic Sequencing Approach to Close Endosymbiont Genomes of Low-Biomass Insect Populations.

With the current advancements in DNA sequencing technology, the limiting factor in long-read metagenomic assemblies is now the quantity and quality of input DNA. Although these requirements can be met through the use of axenic bacterial cultures or large amounts of biological material, insect systems that contain unculturable bacteria or that contain a low amount of available DNA cannot fully utilize the benefits of third-generation sequencing. The citrus greening disease insect vector is an example that exhibits both of these limitations.

Growth parameters of Liberibacter crescens suggest ammonium and phosphate as essential molecules in the Liberibacter-plant host interface.

Background: Liberibacter crescens is the closest cultured relative of four important uncultured crop pathogens. Candidatus. L.

Development of Chemically Defined Media Reveals Citrate as Preferred Carbon Source for Growth.

is the closest cultured relative of four important uncultured crop pathogens. L. asiaticus, L.