Biochip data normalization using multifunctional probes.

Using a biochip with stable probe functionalization and a detection system capable of real time measurements, it is demonstrated that acquired probe-target interaction data are more reproducible in time--on a given probe spot using sequential target runs--than in space, using many probe spot replicates on the biochip in one single parallel target run. To increase the biochip data precision, a normalization method that quantifies and corrects the surface inhomogeneity without the use of complex data post-processing has been developed. This simple and effective method is based on adding a common reactive group to all probes and quantifying the biochip response to a calibration target, thus quantifying the spatial heterogeneity in the biosensor responsiveness.

Polarimetric surface plasmon resonance imaging biosensor.

We report the realization of a polarimetric surface plasmon resonance imaging system capable of dynamically resolving a change in the optical anisotropy of biochemical films. Anisotropies as small as 10(-3) refractive index unit on nanometer-thick samples can be resolved. As an example, we present here the dynamical anisotropy obtained by the electrical patterning of a film consisting of a self-assembled monolayer deposited on gold, covered with a phospholipid hemimembrane.

Surface plasmon resonance spectro-imaging sensor for biomolecular surface interaction characterization.

Surface plasmon resonance (SPR) techniques have become, over the last ten years, powerful tools to study biomolecular surface interaction kinetics in real-time without any use of labels. The highest resolution is currently obtained using spectroscopic SPR systems through the measurement of the complete surface plasmon resonance curve in angular or spectral configuration. But, these systems are limited to a few independent channels (<10).