Effect of saccharides on secondary compounds production from stem derived callus of .

is a subshrub plant known for its toxicity which results from the presence of the tropane alkaloid scopolamine and hyoscyamine. Saccharides are one of the most important elicitors that can alter physiological and biochemical responses in plants. This study targeted to increase the production of secondary metabolites in avoiding genetic alteration and processes using different nontoxic and biodegradable compounds, utilizing calli induced from stems to observe the effects of mannitol and sorbitol on the production of the two major secondary metabolites, scopolamine and hyoscyamine.