Fecal sphingolipids predict parenteral nutrition-associated cholestasis in the neonatal intensive care unit.

Background: Parenteral nutrition-associated cholestasis (PNAC) in the neonatal intensive care unit (NICU) causes significant morbidity and associated healthcare costs. Laboratory detection of PNAC currently relies on elevated serum conjugated bilirubin levels in the aftermath of impaired bile flow. Here, we sought to identify fecal biomarkers, which when integrated with clinical data, would better predict risk for developing PNAC.

Serum malondialdehyde as a lipid peroxidation marker in multiple sclerosis patients and its relation to disease characteristics.

Introduction Oxidative stress has been suggested to play a key role in pathogenesis of multiple sclerosis (MS), but clinical data on oxidative stress markers in MS patients and their influence on clinical and radiologic characteristics of the disease are inconsistent. The aim of this study is to assess the serum levels of malondialdehyde (MDA) as a measure of lipid peroxidation in MS patients and its relation to disease characteristics. Methods This case control study included 120 patients with clinically definite relapsing remitting multiple sclerosis (RRMS) compared to 120 age and sex -matched healthy controls.

An ambient-temperature storage and stabilization device performs comparably to flash-frozen collection for stool metabolomics in infants.

Background: Stool metabolites provide essential insights into the function of the gut microbiome. The current gold standard for storage of stool samples for metabolomics is flash-freezing at - 80 °C which can be inconvenient and expensive. Ambient temperature storage of stool is more practical, however no available methodologies adequately preserve the metabolomic profile of stool.