In vitro and in vivo evaluation of efficacy of citrate/methylene blue/parabens/IPA solution as a skin disinfectant.

Objective: The objective of this study was to evaluate the bactericidal activity of a new antiseptic in an in vitro model using reference bacterial strains and on abdominal and inguinal skin of healthy human subjects. ZuraPrep (C/MB/P/IPA) contains citrate, methylene blue, parabens and isopropyl alcohol 70%.

Methods: In vitro and in vivo studies evaluate immediate and persistent activity of the tested solution using bovine rawhides inoculated with single strains of microorganisms and natural bacterial flora on skin of adult human subjects.