Lens culinaris β-galactosidase (Lsbgal): Insights into its purification, biochemical characterization and trisaccharides synthesis.

Present work describes the purification of an acidic β-galactosidase from Lens culinaris (Lsbgal) to homogeneity via 857 fold with specific activity of 87 U/mg. The molecular mass of purified Lsbgal was estimated ~ 76 kDa by Size Exclusion Chromatography on Superdex-200 (ÄKTA purifier) and on SDS-PAGE, showed hetero-dimeric subunits i.e.