[Designing of hybrid human interferon alfa-2 strain-producers and the use of enteropeptidase for obtaining N-terminal methionine-free interferons].

A system for production of human interferon-alpha2a (IFN-alpha2a) and IFN-alpha2b lacking N-terminal methionine has been developed. Plasmids containing genes of hybrid IFN-alpha2 under the control of different promoters were constructed; a sequence encoding the enteropeptidase hydrolysis site being introduced in proximal part of the genes. As the result, 4 strains of Escherichia coli producing hybrid IFN-alpha2 have been obtained.

[Effect of the nutrient medium composition on biotin biosynthesis by an Actinomyces species 313-152 culture].

The effect of the composition of the nutrient medium on the biosynthesis of biotin by the culture Actinomyces species 313-152 was investigated. This microorganism can synthesize biotin on simple and complex media. The biotin synthesis is stimulated by oleic acid and Tween-80 utilized by the culture as a carbon source.