Production and stably maintenance of strigolactone by transient expression of biosynthetic enzymes in .

Strigolactones (SLs) are phytohormones that play an essential role in plant-microbe interactions. The instability of SLs makes it challenging to use them for application to agriculture. In this study, we successfully produced a large amount of the 4-deoxyorobanchol (4DO), one of SLs, in the leaves of , using a transient expression system to express SL biosynthetic enzymes.

High-Yield Production of the Major Birch Pollen Allergen Bet v 1 With Allergen Immunogenicity in .

Type I allergy is an immunological disorder triggered by allergens and causes significant health problems. The major allergen of birch pollen is Bet v 1, which belongs to the pathogen-related protein 10 (PR-10) family. Here, we established a rapid and robust method for the production of Bet v 1 in leaves, with binding activity to allergic patients' IgE.