Publications by authors named "Akira Shimotsuma"
Article Synopsis
- - Dead cells release various biological molecules, and two main types of cell death—necrosis and apoptosis—play significant roles in cancer development.
- - The study focused on mouse tumor cells treated with the anticancer drug floxuridine (FUdR), finding that the original strain experienced necrosis while a variant underwent apoptosis.
- - Analysis of the secretome (proteins released by cells) revealed that protein leakage contributes to signaling processes related to cell death, identifying specific proteins that may mediate these different cell death mechanisms.
MicroRNAs (miRNAs) are small noncoding RNA molecules that interact with target mRNAs at specific sites to induce cleavage of the mRNA or inhibit translation. Such miRNAs play a vital role in gene expression and in several other biological processes, including cell death. We have studied the mechanisms regulating cell death (necrosis in original F28-7 cells and apoptosis in their variant F28-7-A cells) in the mouse mammary tumor cell line FM3A using the anticancer agent floxuridine (FUdR).
View Article and Find Full Text PDFDirect interaction analysis of microRNA-351-5p and nuclear scaffold lamin B1 mRNA by the cell-free in vitro mRNA/miRNA binding evaluation system.
Nucleosides Nucleotides Nucleic Acids
December 2020
We previously demonstrated that miR-351-5p regulates nuclear scaffold lamin B1 expression and mediates the anticancer floxuridine-induced necrosis shift to apoptosis in mammalian tumor cells. Notably, it is unknown whether lamin B1 mRNA is a direct target of miR-351-5p. Here, we show that miR-351-5p interacts with a lamin B1 mRNA partial sequence by using the cell-free miRNA and mRNA binding evaluation system.
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