Isolation and characterization of antagonistic fungi against potato scab pathogens from potato field soils.

Potato scab is a serious plant disease caused by several Streptomyces sp., and effective control methods remain unavailable. Although antagonistic bacteria and phages against potato scab pathogens have been reported, to the best of our knowledge, there is no information about fungi that are antagonistic to the pathogens.

Development of a genotyping method for potato scab pathogens based on multiplex PCR.

Scab disease significantly damages potato and other root crops. Streptomyces scabiei, S. acidiscabiei, and S.

Catellibacterium nectariphilum gen. nov., sp. nov., which requires a diffusible compound from a strain related to the genus Sphingomonas for vigorous growth.

A bacterial strain, designated AST4(T), was isolated from activated sludge. The bacterium did not show significant growth on nutrient broth, but growth was clearly stimulated by addition of supernatant from other bacterial cultures. Culture filtrate of a strain related to the genus Sphingomonas in particular increased the cell yield and growth rate of strain AST4(T).

Flow cytometric sorting, phylogenetic analysis and in situ detection of Oscillospira guillermondii, a large, morphologically conspicuous but uncultured ruminal bacterium.

Flow cytometric sorting based on its large cell size enabled an enriched fraction of Oscillospira guillermondii cells to be obtained from the rumen contents of a sheep. Phylogenetic analysis based on cloned 16S rDNA sequences indicated that the bacterium is a member of the low-G+C Gram-positive bacterial cluster. Sporobacter termitidis and Papillibacter cinnamivorans were the most closely related known species, with sequence similarities of only 86.

The ratio of L-form to D-form of lactic acid as a criteria for the identification of lactic acid bacteria.

Stereoisomers of lactic acid produced by lactic acid bacteria were determined by HPLC by using an enantiomeric resolution column. In general, the ratios of L-form to D-form (the type of stereoisomer) obtained were compared with those in references. Values of the type of stereoisomer of lactic acid were discussed from the viewpoint of identification of lactic acid bacteria.

Effects of sodium acetate on the production of stereoisomers of lactic acid by Lactobacillus sakei and other lactic acid bacteria.

Lactobacillus sakei and other lactic acid bacteria were studied on the change of the type of stereoisomers (the ratio of L-form to D-form) of lactic acid produced in the presence of sodium acetate and under other cultural conditions. Of 49 strains tested, only L. sakei NRIC 1071(T) and L.