Synthetic Genetic Elements Enable Rapid Characterization of Inorganic Carbon Uptake Systems in H16.

H16 is a facultative chemolithotroph capable of using CO as a carbon source, making it a promising organism for carbon-negative biomanufacturing of petroleum-based product alternatives. In contrast to model microbes, genetic engineering technologies are limited in , constraining its utility in basic and applied research. Here, we developed a genome engineering technology to efficiently mobilize, integrate, and express synthetic genetic elements (SGEs) in .