Visualization of the localization of phospholipids in developing rat teeth by matrix-assisted laser desorption/ionization imaging mass spectrometry.

Matrix-assisted laser desorption/ionization imaging mass spectrometry (IMS) is used to comprehensively visualize the spatial distribution of numerous biomolecules. The present study was designed to investigate the distribution of phospholipids in developing rat teeth by IMS to identify the characteristic phospholipid molecules for tooth development, and to evaluate the suitability of tissue preparation methods. Rats at postnatal day 3 were euthanized, and the resected head specimens were either fixed or not fixed with 4% paraformaldehyde (PFA), and decalcified or not decalcified in 10% ethylenediaminetetraacetic acid (EDTA) before being frozen.

The visualization of the mineral and protein distribution in the same histological sections of rat calcified growth plate cartilage.

Objective: To determine whether the combination of scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) and immunostaining would visualize the mineral and protein distribution in the same histological sections.

Methods: Paraffin sections of fixed rat hindlimbs were processed for SEM-EDX and subsequently for immunofluorescence staining.

Results: The localization of calcium, phosphorus, and carbon with type II collagen could be visualized in the same region of calcified growth plate cartilage on the same section.

Degradation of extracellular matrices propagates calcification during development and healing in bones and teeth.

Background: Bone, dentin, and enamel are tissues formed through calcification, a process involving deposition of calcium phosphate minerals on extracellular organic matrices. Calcification, the underlying mechanism of which is unknown, is initiated with mineral deposition followed by advancing of the deposit and subsequent maturation of the mineral crystal.

Highlight: We have reviewed the current knowledge of how calcification proceeds during bone development, bone healing, and enamel and dentin development, based on reported studies.

Experimental Comparison of the Performance of Cutting Bone and Soft Tissue between Piezosurgery and Conventional Rotary Instruments.

Piezosurgery is an innovative technique widely used for osteotomies in the field of oral and maxillofacial surgery. The surgical technique has been clinically supposed to cut mineralized bone selectively with reducing the risk of damage to adjacent soft tissues. However, none of the previous literature has reported any evidence of scientific experiments to examine performance of the piezoelectric device, i.

Calcification in rat developing mandibular bone demonstrated by whole mount staining, microcomputed tomography and scanning electron microscopy with energy dispersive X-ray spectroscopy.

The study was designed to investigate calcification in developing rat mandibular bone using whole mount staining, micro-computed tomography (micro-CT) and scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX). Wistar rats at embryonic days 16, 18, and 20 and postnatal weeks 1 and 6 were used. Rats were fixed with 4% paraformaldehyde and heads were resected, frozen and sectioned for histology, then analysed with SEM-EDX.

Analysis of calcium, phosphorus, and carbon concentrations during developmental calcification of dentin and enamel in rat incisors using scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX).

Objective: The study was designed to investigate the concentrations of calcium (Ca), phosphorus (P), and carbon (C) during developmental calcification of dentin and enamel in rat incisors.

Methods: Mandibular incisors from eight 2-week-old male Wistar rats were analyzed by scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX). We analyzed data on the elements in the course of developmental processes in dentin and enamel and along the vertical line of the matrix between odontoblasts and ameloblasts.

Bone matrix calcification during embryonic and postembryonic rat calvarial development assessed by SEM-EDX spectroscopy, XRD, and FTIR spectroscopy.

Bone mineral is constituted of biological hydroxyapatite crystals. In developing bone, the mineral crystal matures and the Ca/P ratio increases. However, how an increase in the Ca/P ratio is involved in maturation of the crystal is not known.

Involvement of sensory neurons in bone defect repair in rats.

We investigated bone repair in sensory-denervated rats, compared with controls, to elucidate the involvement of sensory neurons. Nine-week-old male Wistar rats received subcutaneous injections of capsaicin to denervate sensory neurons. Rats treated with the same amount of vehicle served as controls.