Publications by authors named "Akihiro Kunisawa"
Unlabelled: Over 170 types of chemical modifications have been identified in cellular RNAs across the three domains of life. Modified RNA is eventually degraded to constituent nucleosides, and in mammals, modified nucleosides are released into the extracellular space. By contrast, the fate of modified nucleosides in bacteria remains unknown.
View Article and Find Full Text PDFSARS-CoV-2 infection alters cellular RNA content. Cellular RNAs are chemically modified and eventually degraded, depositing modified nucleosides into extracellular fluids such as serum and urine. Here we searched for COVID-19-specific changes in modified nucleoside levels contained in serum and urine of 308 COVID-19 patients using liquid chromatography-mass spectrometry (LC-MS).
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- Peak picking is essential in chromatography for identifying the start and end points of peaks, but accurately labeling these points has been challenging due to overlaps in real data.
- A new method has been developed to generate fake chromatograms that include clearly defined peak boundaries, allowing for better training of deep learning neural networks.
- The resulting peak-picking neural networks exceeded the performance of traditional software and matched the accuracy of skilled operators, highlighting the importance of these generated fake chromatograms for future advancements in peak picking technology.
D-amino acids (D-AAs) have various biological activities, such as activation of N-methyl-D-aspartic acid (NMDA) receptor as a co-agonist by D-Ser. Since several free D-AAs are released in the broth monocultured with bacterium and D-AAs are probably utilized for bacterial communication, we presume that intestinal microbiota releases several kinds of free D-AAs, which may be involved in the hosts' health. However, presently, only four free D-AAs have been found in the ceacal lumen, but not in the colonic lumen.
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