Novel PCR-based genotyping method, using genomic variability between repetitive sequences of toxigenic Vibrio cholerae O1 El Tor and O139.

A novel genotyping method for toxigenic Vibrio cholerae O1 El Tor and O139 was developed. The method was designed to amplify DNA sequences "sandwiched" between any given pair of repetitive sequences, "V. cholera repeats (VCR)", in highly polymorphic "integron island" of ca.

Genetic analyses of the putative O and K antigen gene clusters of pandemic Vibrio parahaemolyticus.

Pandemic V. parahaemolyticus strains have rapidly changed their serotypes, but its determinants, especially K antigen, and the genes involved in serotype have been an open question. The purpose of this study was to gain insights into these points.

Identification of enterohemorrhagic Escherichia coli O157-Specific DNA sequence obtained from amplified fragment length polymorphism analysis.

An approximately 1.1 kbp fragment that was commonly observed only in the enterohemorrhagic Escherichia coli (EHEC) O157 strains in an analysis of amplified fragment length polymorphism was found to be a partial gene sequence encoding the locus of toxB and a useful molecular marker for the identification of EHEC O157.