[Establishment of simultaneous measurement method of 8 salivary components using urinary test paper and clinical evaluation of oral environment].

Purpose: Clinical findings were compared with glucose, protein, albumin, bilirubin, creatinine, pH, occult blood, ketone body, nitrite, and white blood cells contained in whole saliva to investigate the components that most markedly reflect the periodontal condition.

Material And Method: The subjects were staff of the Prosthodontics Department, Showa University, and patients who visited for dental treatments (57 subjects in total). At the first time, saliva samples were gargled with 1.

Identification and characterization of the precursors committed to osteoclasts induced by TNF-related activation-induced cytokine/receptor activator of NF-kappa B ligand.

Osteoclasts are terminally differentiated from cells of monocyte/macrophage lineage by stimulation with TNF-related activation-induced cytokine (TRANCE) (receptor activator of NF-kappaB ligand/osteoprotegerin ligand/osteoclast differentiation factor/TNFSF11/CD254). In the present study, we attempted to determine when and how the cell fate of precursors becomes committed to osteoclasts following TRANCE stimulation. Although mouse bone marrow-derived macrophages (BMMs) were able to differentiate into either osteoclasts or dendritic cells, the cells no longer differentiated into dendritic cells after treatment with TRANCE for 24 h, indicating that their cell fate was committed to osteoclasts.

[Differences in whole salivary total protein concentration and protein fractions among the groups of dentulous subjects, edentulous subjects and periodontitis patients].

Purpose: To apply salivary proteins to the diagnosis of periodontal disease in partial denture supporting teeth, total protein concentrations and protein fractions in whole saliva were compared among dentulous subjects, edentulous subjects and periodontitis patients.

Materials And Methods: Eighty-five subjects/patients in total were studied, who consisted of 52 dentulous subjects with diagnosed normal periodontal tissue, 18 edentulous subjects using complete dentures and 15 patients with diagnosed periodontal disease. Total protein concentration in whole saliva was measured using dot blotting-silver staining, and protein fractions were analyzed using cellulose acetate membrane electrophoresis and silver staining.