RNA-Seq explores the functional role of the fibroblast growth factor 10 gene in bovine adipocytes differentiation.

Objective: The present study was executed to explore the molecular mechanism of fibroblast growth factor 10 (FGF10) gene in bovine adipogenesis.

Methods: The bovine FGF10 gene was overexpressed through Ad-FGF10 or inhibited through siFGF10 and their negative control (NC) in bovine adipocytes, and the multiplicity of infection, transfection efficiency, interference efficiency were evaluated through quantitative real-time polymerase chain reaction, western blotting and fluorescence microscopy. The lipid droplets, triglycerides (TG) content and the expression levels of adipogenic marker genes were measured during preadipocytes differentiation.

In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study.

In vitro fertilization (IVF) technologies have great potential in the preservation of endangered species. In the current study, an IVF experiment was carried out to evaluate whether reproductive technologies are suitable for Kazakh Whiteheaded cattle, aimed at preserving this breed whose population has reduced drastically over the last thirty years. The reproduction characteristics of Kazakh Whiteheaded cows were compared to Aberdeen Angus cows.

Knowledge, attitude and practice (KAP) of smallholder farmers on foot-and-mouth disease in Cattle in West Kazakhstan.

Background: This study was performed to assess the knowledge, attitudes and practices (KAPs) of farmers and veterinary professionals towards foot-and-mouth disease (FMD) in the area studied.

Methods: The study was based on a comprehensive questionnaire administered through face-to-face interviews. Between January and May 2022, 543 households and 27 animal health practitioners (AHP) were visited in 4 provinces of the West Kazakhstan region to assess their KAPs towards FMD.