Publications by authors named "Akemi Okubo"
Article Synopsis
- - The text discusses advancements in gene function analysis using gene knockout methods in mice, particularly highlighting the limitations of traditional conditional gene knockout strategies that may take too long to observe phenotypes due to the stability of target proteins.
- - A new technique using an improved auxin-inducible degron system, AID2, allows for rapid and reversible protein knockdown through a simple injection, which can lead to observable effects within hours rather than days.
- - The researchers developed several specific mouse lines to utilize the AID2 system and successfully demonstrated the effective depletion of an endogenous protein, DCP2, using gene editing, resulting in similar phenotypes to those seen in traditional knockout models.
The transcriptome dynamically changes via several transcriptional and post-transcriptional mechanisms. RNA-binding proteins contribute to such mechanisms to regulate the cellular status. DDX6 is one such protein and a core component of processing bodies (P-bodies), membrane-less cytosolic substructures where RNA and proteins localize and are functionally regulated.
View Article and Find Full Text PDFThe metameric structure in vertebrates is based on the periodic formation of somites from the anterior end of the presomitic mesoderm (PSM). The segmentation boundary is defined by the Tbx6 expression domain, whose anterior limit is determined by Tbx6 protein destabilization Ripply2. However, the molecular mechanism of this process is poorly understood.
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