Vitamin B Enhances the Cytotoxicity of Diethyldithiocarbamate in a Synergistic Manner, Inducing the Paraptosis-Like Death of Human Larynx Carcinoma Cells.

We have shown that hydroxycobalamin (vitamin В) increases the toxicity of diethyldithiocarbamate (DDC) to tumor cells by catalyzing the formation of disulfiram (DSF) oxi-derivatives. The purpose of this study was to elucidate the mechanism of tumor cell death induced by the combination DDC + В. It was found that cell death induced by DDC + B differed from apoptosis, autophagy, and necrosis.

Generation of recombinant porcine parvovirus virus-like particles in Saccharomyces cerevisiae and development of virus-specific monoclonal antibodies.

Porcine parvovirus (PPV) is a widespread infectious virus that causes serious reproductive diseases of swine and death of piglets. The gene coding for the major capsid protein VP2 of PPV was amplified using viral nucleic acid extract from swine serum and inserted into yeast Saccharomyces cerevisiae expression plasmid. Recombinant PPV VP2 protein was efficiently expressed in yeast and purified using density gradient centrifugation.

[State of crural perforating veins after sclerosurgical interventions for lower limb varicose disease].

Analysed herein are the findings obtained by comprehensive examination of 106 patients presenting with grade C3 chronic venous insufficiency according to the CEAP classification. Localization, intensity and extent of refluxes of blood in the venous system of the affected extremity were determined by means of ultrasonographic angioscanning. Based on the obtained results, the patients were subdivided into two groups.

[Comparative assessment of efficacy of local thrombolysis with various fibrinolytics].

To avoid the characteristic for thrombolytic therapy of pulmonary embolism problems associated with low efficacy and elevated danger haemorrhagic complications may, in our opinion, be possible by means of the method of local thrombolysis worked out in our Clinic (RF Patent No 2376042). The present work was aimed at assessing efficacy of local thrombolysis in patients diagnosed as having pulmonary artery embolism with the drugs urokinase and actilyse. We examined a total of twenty patients.

[Role of blood refluxes in the genesis of venous trophic disorders in patients with chronic venous insufficiency].

The authors analyzed the results of a comprehensive examination of 120 patients with class C6 chronic venous insufficiency according to the CEAP classification, and open trophic ulcers. The patients were subdivided into 2 groups. Group One comprised a total of 75 patients with varicosity, and Group Two consisted of 45 patients with post-thrombotic disease.

[Effects of blood refluxes on formation of trophic ulcers in patients with postthrombotic disease].

The results of complex examination of 45 patients with chronic venous insufficiency having class C6 open trophic ulcers by CEAP classification due to postthrombotic disease were analyzed. The localization, intensity and duration of blood refluxes in the venous system of the injured extremity were determined using ultrasonic angioscanning. Highly intensive total reflux of blood in the popliteal and talocrural segments of the lower extremity venous system found in 68.

[Hemodynamic characteristics and priority of blood refluxes in genesis of trophic ulcers in patients with varicose disease of lower extremities].

An analysis of results of complex examination of 75 patients with varicose disease and trophic ulcers has shown that in 81.4% of the patients the main hemodynamic factor resulting in the development of ulcers was highly intensive total-subtotal reflux of blood along the superficial veins, and in 9.3% it was the highly intensive profound reflux along the posterior tibial veins.

[Enzyme immunoassay system for detection of staphylococcal enterotoxin, type C].

An enzyme immunoassay (EIA) system for detection of staphylococcal enterotoxin, type C, has been developed. The sensitivity of the system is 1 ng/ml. The optimum EIA parameters have been worked out.

[Correction of immune response using purified staphylococcal toxoid and likopid in the secondary immunodeficiency induced by Coxsackie virus B3].

The action of immunomodulators, purified staphylococcal toxoid (PST) and lycopid, on secondary immunodeficiency state developing during infection caused by Coxsackie virus B3 was studied. This defect was manifested by delayed hypersensitivity to sheep red blood cells (SRBC) and the suppression of neutralizing antibodies to poliomyelitis virus. Depending on the scheme of the experiment, PST normalized the defects of immune response to SRBC or poliovirus, increased suppression or showed no activity.

[Detection of Staphylococcus aureus and Staphylococcus epidermis species specific antigens and antibodies to alpha-toxin in the blood of patients with pneumonia].

Test systems for indirect hemagglutination (IHA) test for detection of S. aureus and S. epidermidis teichoic acids and S.

[Improvement of epidural anesthesia in reconstructive surgery of the aorta in Leriche's syndrome].

Fifty-six patients subjected to aortofemoral bilateral shunting under prolonged epidural anesthesia are examined. Swan-Gans catheter was used to evaluate central and pulmonary hemodynamics. Ganglionic blocking with pentamine for taxiphylaxis was carried out in 31 patients prior to catheterization of the epidural space.

[A system of step-by-step differentiation of methicillin-resistant Staphylococcus aureus].

One hundred and eighty four strains of methicillin resistant Staphylococcus aureus (MRSA) isolated in pyosurgical and burn departments of Moscow, Minsk, Omsk, Tbilisi, Vologda, Smolensk and Dushanbe were differentiated with using phages of the International Set (BIS), two collections of experimental phages and two-probe fingerprinting. More than 50 per cent of the isolates could not be typed by the BIS phages. The Experimental Collection of the N.

[The development of species-specific test systems for the rapid diagnosis of staphylococcal infections].

Highly purified teichoic acids (TA) were isolated from Staphylococcus aureus and S. epidermidis and characterized. The preparations of TA were highly species-specific.

[Additional differentiation of methicillin resistant strains of Staphylococcus aureus typed by the international phage bank].

In the methicillin resistant strains of Staphylococcus aureus (MRSA) typed by the International Set phages the host specificity of the restriction-modification of the phage 85 DNA was determined, the finger printing of the cell DNA was carried out with using two probes and the lytic spectrum of the phages induced in them was studied. Four clones with different specificity of the restriction-modification system (rm89, rm108, rm121 and rm947) differing from that of strain PS 85 which is the host of phage 85 were detected. The strains belonging to the modification types m89, m108 and m121 contained prophages (within the respective groups) with similar lytic spectra when tested with the use of the PS strains of the International Collection and had cross antiphage immunity.

[The patterns of the corrective action of a natural immunomodulator on the secondary immunological defect caused by a corpuscular pertussis vaccine].

Purified staphylococcal toxoid (PST) was shown to be capable of preventing the development of secondary antigen-nonspecific immune deficiency in mice, immunized with whole-cell pertussis vaccine. The immunocorrective action of PST was manifested after its injection before (on day -1), simultaneously with and after (on day +1) the injection of whole-cell pertussis vaccine. Correction was either complete or partial, depending on the scheme of the experiment and the dose of PST.

[The modulation of cellular immunity in vivo and in vitro under the action of purified staphylococcal anatoxin].

Purified staphylococcal toxoid modulates (mainly suppresses) cell-mediated immune response to heterogeneous antigens of animal origin (to sheep red blood cells as shown by the delayed hypersensitivity reaction) or bacterial origin (to BCG as shown by the splenocyte migration test). The direction and manifestation of modulation depend on the strain of mice, dose of the toxoid, dose and nature of the test antigen and the immunization schedule (intervals between the injections of the antigen).

[The capacity of purified staphylococcal anatoxin to correct antigen-specific and antigen-nonspecific immunological defects].

Purified staphylococcal toxoid is capable of partially preventing the development of antigen-specific (induced by the supraoptimal dose of sheep red blood cells) and antigen-nonspecific (induced by Tahyna virus) defects of humoral immune response, as well as abolishing these defects. The presence and manifestation of the correction of virus-induced immunodeficiency is determined by the dose of the toxoid and the interval between the injections of purified staphylococcal toxoid and the infective agent.

Lysogeny of methicillin-resistant Staphylococcus aureus and the role of prophages in transfer of conjugative and non-conjugative plasmids.

The lysogenicity of 49 strains of methicillin-resistant S. aureus (MRSA) isolated in Moscow clinics in the 1970s and '80s was studied by the method of mitomycin C induction. It was found that one strain had phage of serogroup B, 33 strains had serogroup F phages and 15 strains had phages of both serogroups.

A new approach to establishing the set of phages for typing methicillin-resistant Staphylococcus aureus.

A new approach to using experimental phages for typing methicillin-resistant S. aureus (MRSA) non-sensitive to the phages of International Basic Set (IBS) is described. The collection Includes phage 85, modified on a culture of MRSA, and 5 phages induced from MRSA strains isolated in clinics of Moscow in 1975-76.