Simultaneous determination of intracellular reduced and oxidized glutathiones by the König reaction.

The König reaction is commonly used for the detection of cyanide and its derivatives, including thiocyanate and selenocyanate. We found that this reaction can be used to quantify glutathione fluorometrically, and applied it to the simultaneous determination of reduced and oxidized glutathiones (GSH and GSSG) using a conventional LC system with isocratic elution. The limits of detection were 6.

Outcomes of combined gonioscopy-assisted transluminal trabeculotomy and goniosynechialysis in primary angle closure: a retrospective case series.

Purpose: To evaluate the additional intraocular pressure (IOP) lowering effect of gonioscopy-assisted transluminal trabeculotomy (GATT) to contemporary goniosynechialysis (GSL) in endeavouring to abolish subsequent occlusion after chronic iridotrabecular contact in primary angle closure (PAC) patients.

Methods: A retrospective case series of all PAC eyes underwent GATT + GSL with or without phacoemulsification and intraocular lens implantation (PEA + IOL) from December 2016 to May 2018 were recruited. IOP and the number of anti-glaucoma medications were compared pre- and post-operatively by Wilcoxon signed-rank test.

Photoluminescent Detection of Nitrite with Carbon Nanodots Prepared by Microwave-assisted Synthesis.

A photoluminescent detection method for nitrite with high selectivity and sensitivity using carbon nanodots (CNDs) is demonstrated. The selectivity of nitrite is accomplished by a highly specific diazotization reaction between nitrite and p-phenylenediamine (p-PDA). In the presence of nitrite, p-PDA easily reacts to form the diazonium cation in the acidic aqueous solution.

Morphological and histopathological changes in orofacial structures of experimentally developed acromegaly-like rats: an overview.

Tongue enlargement and mandibular prognathism are clinically recognized in almost all patients with acromegaly. An acromegaly-like rat model recently developed by exogenous administration of insulin-like growth factor I (IGF-I) was used to investigate morphological and histopathological changes in orofacial structures and to clarify whether these changes were reversible. Exogenous administration of IGF-I evoked specific enlargement of the tongue with identifiable histopathological changes (increased muscle bundle width, increased space between muscle bundles, and increased epithelial thickness), elongation of the mandibular alveolar bone and ascending ramus, and lateral expansion of the mandibular dental arch.

Evaluation of superior BACE1 cleavage sequences containing unnatural amino acids.

A recombinant form of BACE1 (β-site amyloid precursor protein cleaving enzyme-1) corresponding to positions 46-454 of the extracellular domain of the original membrane enzyme was prepared. The recombinant BACE1 (rBACE1) had the kinetic parameters K(m)=5.5μM and k(cat)=1719s(-1).

Patient perceptions of different lancing sites for self-monitoring of blood glucose: a comparison of fingertip site with palm site using the OneTouch Ultra Blood Glucose Monitoring System.

Background: Alternate-site testing (AST) for self-monitoring of blood glucose leads to improved glycemic control for treatment of diabetes mellitus. The aim of this randomized, open-label, two-period, crossover study was to compare the comfort of two different lancing sites, fingertip and palm sites, for AST in diabetes patients.

Methods: Patients injected insulin four times a day.

Excessive lateral dental arch expansion in experimentally developed acromegaly-like rats.

Objective: To investigate morphological changes in the dental arches of acromegaly-like rats recently developed by means of exogenous IGF-I administration.

Design: Human recombinant IGF-I (640microg/day) was continuously administered subcutaneously for 4 weeks by osmotic mini-pumps to 10-week-old male rats (n=6). Control animals were injected with saline alone (n=6).