Publications by authors named "Akalan H"
Article Synopsis
- This study investigated the effects of lopinavir/ritonavir (Lop/r) on intervertebral disc (IVD) health, aiming to understand its potential in slowing down IVD degeneration and reducing inflammation.
- Results showed that Lop/r treatment decreased the proliferation of IVD cells but did not cause cell death; it also led to significant reductions in inflammation markers IL-1β and NF-κB while increasing positive indicators like SOX9.
- Findings suggest that Lop/r could have promising applications in treating IVD degeneration, although more research is needed to confirm these results in clinical settings.
Purpose: The aim of this study was to establish whether Acetobacter ghanensis, the probiotic characteristics of which were evaluated previously, attenuates gliadin-induced toxicity in intestinal epithelial cells with gluten-digestive and immunoregulatory properties.
Methods: A co-culture model of human intestinal epithelial cell (Caco-2) monolayers on top of peripheral blood mononuclear cells (PBMCs) obtained from patients with celiac disease (CD) was established. The gluten-digestive properties of A.
Article Synopsis
- Recent studies highlight inflammation as a key factor in intervertebral disc degeneration (IVDD) and osteoarthritis (OA), prompting this research to explore the effects of the anti-inflammatory drug oseltamivir on IVD and cartilage cells.
- The study involved isolating human lumbar IVD and cartilage tissues to assess cell viability and changes in important proteins (HIF-1α, IL-1β, NF-κB, and NLRP3 inflammasome) after oseltamivir treatment.
- Results showed that oseltamivir reduced cell proliferation and significantly altered protein expressions linked to inflammation and cellular health, suggesting its potential as a regenerative treatment for IVDD and OA.
Objective: The study aimed to examine the effects of two drugs, an acetylcholinesterase inhibitor (AChEI) and an N-methyl-D-aspartate receptor (NMDAR) antagonist, on degenerated annulus fibrosus (AF) and nucleus pulposus (NP) cells and the extracellular matrix (ECM) structure in vitro.
Patients And Methods: Tissue samples were obtained from patients with intervertebral disc herniation (four males and four females; classified as Pfirmann stage IV) and used to prepare cell cultures. Untreated cell culture samples served as the control group.
In addition to alloantibodies, alloreactive memory B cell (mBC) evaluation has a potential for immunological risk assessment during transplantation processes. For the alloreactive mBCs evaluation currently, direct Flow Cytometric (FC) analysis using the HLA tetramer staining is an option. Evaluation of alloantibodies produced by the polyclonally stimulated alloreactive mBCs in in vitro culture system seems to be another useful approach, but this needs further downstream applications.
View Article and Find Full Text PDFAim: To evaluate the effects of favipiravir (FVP) on cell viability and cytotoxicity in human degenerated primary intervertebral disc (IVD) tissue cell cultures. Furthermore, the protein expressions of hypoxia-inducible factor 1 alpha (HIF-1α), nuclear factor-kappa-b (NF-kB), and interleukin-1 beta (IL-1β) were also examined.
Material And Methods: Untreated cell cultures served as the control group, named group 1.
Introduction: The present study aimed to investigate the potential effects of rivaroxaban, an oral anticoagulant that inhibits the effects of factor Xa, on intact intervertebral disc tissue cells and the extracellular matrix (ECM).
Material And Methods: Rivaroxaban was applied to primary human cell cultures prepared from tissues of the intervertebral disc. Comparative molecular analyses were performed on non-drug-treated control group samples.
Aim: To investigate the effects of metformin, a drug used widely for the treatment of type 2 diabetes mellitus, on human primary cell cultures prepared from uninjured segment of disc material intervertebral disk tissues.
Material And Methods: Primary cell cultures were prepared using the tissues of six patients (three males and three females) who had undergone lumbar microdiscectomy and sequestrectomy. Untreated samples served as the control group, and metformintreated samples served as the experimental group.