[The volatile fatty acids in children with dysfunction of biliary tract].

The volatile fatty acids are metabolites of bacteria reflecting condition and disbiotic alterations of microflora of gastrointestinal tract. The study was carried out to determine qualitatively volatile fatty acids in saliva of children with dysfunction of biliary tract and healthy ones. The indices of volatile fatty acids were analyzed in 46 children aged 7-17 years and with dysfunction of biliary tract.

[VOLATILE FATTY ACIDS IN SALIVA--BIOLOGICAL MARKERS FOR ASSESSMENT OF DRINKING WATER POLLUTANTS ON CHILDREN].

The use of modern methods of analysis is aimed to the search of ultimately novel biological markers. Volatile fatty acids in saliva were not used previously for the assessment of the effects of contaminating substances in the drinking water on the body of children. The aim of the study is to investigate the informative value of volatile fatty acids in saliva as biological markers of the impact for the assessment of the exposure to contaminating substances in the drinking water on the body of children.

[Outpatient diagnosis of oxidant stress in patients with carbohydrate metabolism disturbance in metabolic syndrome].

Aim: To compare lipid peroxidation (LPO), (NO)-endothelium system in disorders of glucose tolerance (DGT) in patients with metabolic syndrome (MS) and diabetes mellitus type 2 (DM2).

Material And Methods: The trial included 178 patients with DGT and DM2 within MS (WHO, 1999). The control group consisted of 40 healthy subjects free of MS.

[The role of oleic acid in the process of induced destruction of Escherichia coli M-17].

The influence of oleic acid on the process of the destruction of E. coli, induced by the transfer of the bacteria from the growth medium into solution without nutrient substances and their treatment with oleic acid at a temperature of 45 degrees C, was studied. Oleic acid at a concentration of 200 nmol/ml accelerated the destruction of E.

[Changes in the cell size of Escherichia coli M-17 during induced destruction].

The autolysis of E. coli, induced by their deprivation of nutrition in combination with the action of oleic acid and a temperature of 45 degrees C, was studied. The study revealed that an increase in the number of cells during 4 hours after the induction of the process was accompanied by a decrease in their size and an increase in the surface/volume ratio.

[The possibility of using the data on the content of volatile fatty acids for the indirect demonstration of the causative agents of suppurative-inflammatory diseases in the pathological material].

The use of the quantitative content of volatile fatty acids as marker for the indication of the causative agents of pyo-inflammatory diseases in material taken from wounds is proposed. Thus, in 71% of cases the detection of acetic acid in a purulent wound coincided with the bacteriological isolation of monocultures, as well as associations, of facultative anaerobic bacteria. The analysis of isobutyric, butyric, isovaleric, valeric and acetic acids permitted the rapid detection of associations of obligate and facultative anaerobic bacteria in an individual patient.

[Trend in rapid diagnosis of purulent infections pathogens and rapid assessment of treatment efficacy].

A method for rapid diagnosis of wound infection based on gas chromatographic detection of metabolites of anaerobic bacteria is proposed. If acetic acid is present in pathological material from the wounds, bacteriological analysis detects facultative anaerobic bacteria in 71% cases.

[Development of principles for the controlled autolysis of Escherichia and Salmonella].

The autolysis of Escherichia and Salmonella, depending on the conditions of the medium where the cultivation and lysis of the bacteria took place, was studied. The study revealed that the autolysis of these bacteria was most intensive if lysis was induced at the moment when the culture achieved the maximum specific growth rate, as well as in case when the extreme conditions were maintained in the medium during the autolytic process. Approaches to controlling the process of the induced autolysis of Escherichia and Salmonella were proposed and substantiated.

[The effect of the pH of the medium on induced autolysis in populations of enterobacteria].

Changes in pH in the process of the induced autolysis of salmonellae and Escherichia coli were studied. The induced autolysis of enterobacterial populations was studied in connection with the acidity values of the medium in which the process was carried out. The pH of extracellular fluid, the optical density of cell suspension, the content of total protein and amino nitrogen were analyzed.

[Self-regulation of Salmonella minnesota populations in the process of induced autolysis].

The dynamics of the populations of S. minnesota S- and R-forms in autolysis, induced by multifactor methods with and without the use of oleic acid, the analog of autolysis-activating autoregulator, was studied. In the process of autolysis the optical density (OD) of cell suspension, the total number of bacteria, the number of anabiotic refractile cells and the number of colony-forming units (CFU) were analyzed.

[New methods of assessing intoxication and blood cell response in patients with infectious toxicoses].

The microviscosity indexes of cell membranes of erythrocytes, neutrophils, and lymphocytes were estimated in children with acute respiratory diseases with and without infectious toxicosis. The erythrocyte microviscosity most completely reflects the degree of toxicosis. The ratio of membrane microviscosity indexes of neutrophils to that of lymphocytes demonstrates the specific response of blood cells to infectious toxic exposure.

[Heterogeneity of Escherichia coli population during induced autolysis].

Escherichia coli M-17 autolysis was induced by eliminating nutrition sources from the growth medium and exerting a shock with EDTA. The overall cell number, the optical density of the cell suspension, the number of colony-forming units (CFU), and [3H]uracil incorporation into the cells were analysed in the course of autolysis. The number of CFU was found to drop down faster than the overall cell number in the process of autolysis.