Publications by authors named "Ajith Vp"
Article Synopsis
- The mitotic cohesin complex, crucial for sister chromatid cohesion and chromatin structure, associates with chromosomes when DNA double-strand breaks (DSBs) occur.
- A study of meiotic cohesin with Rec8 revealed that its localization changes significantly from the middle to late stages of meiotic prophase I, showing distinct patterns of binding and dissociation.
- The dissociation of Rec8 from chromosomes correlates with the density of DSBs during meiosis, indicating a unique regulatory mechanism that controls Rec8-cohesin binding in response to DSBs specifically during meiosis.
In most sexually reproducing organisms crossing over between chromosome homologs during meiosis is essential to produce haploid gametes. Most crossovers that form in meiosis in budding yeast result from the biased resolution of double Holliday junction (dHJ) intermediates. This dHJ resolution step involves the actions of Rad2/XPG family nuclease Exo1 and the Mlh1-Mlh3 mismatch repair endonuclease.
View Article and Find Full Text PDFCharacterization of systemic genomic instability in budding yeast.
Proc Natl Acad Sci U S A
November 2020
Conventional models of genome evolution are centered around the principle that mutations form independently of each other and build up slowly over time. We characterized the occurrence of bursts of genome-wide loss-of-heterozygosity (LOH) in , providing support for an additional nonindependent and faster mode of mutation accumulation. We initially characterized a yeast clone isolated for carrying an LOH event at a specific chromosome site, and surprisingly found that it also carried multiple unselected rearrangements elsewhere in its genome.
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- The strain JAY270/PE2 is a powerful yeast used for bioethanol production from sugarcane, known for its non-aggregation property which aids in cell recycling during fermentation.
- The strain faces potential genomic instability over time, leading to issues such as cell aggregation and defects in mother-daughter cell separation, resulting in observable rough colonies.
- Investigating these changes revealed that the instability rates in JAY270 are similar to those in conventional lab strains, highlighting that genomic dynamics in this industrial yeast strain are typical rather than excessively unstable.
Mlh1-Mlh3 is an endonuclease hypothesized to act in meiosis to resolve double Holliday junctions into crossovers. It also plays a minor role in eukaryotic DNA mismatch repair (MMR). To understand how Mlh1-Mlh3 functions in both meiosis and MMR, we analyzed in baker's yeast 60 new mlh3 alleles.
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