A Protein in the Yeast Presents DNA Binding Homology to the p53 Checkpoint Protein and Tumor Suppressor.

does not contain a p53 homolog. Utilizing this yeast as an in vivo test tube model, our aim was to investigate if a yeast protein would show p53 DNA binding homology. Electrophoretic mobility shift analyses revealed the formation of specific DNA-protein complexes consisting of nuclear protein(s) and oligonucleotides containing p53 DNA binding sites.

Loss of a 20S proteasome activator in Saccharomyces cerevisiae downregulates genes important for genomic integrity, increases DNA damage, and selectively sensitizes cells to agents with diverse mechanisms of action.

Cytoprotective functions of a 20S proteasome activator were investigated. Saccharomyces cerevisiae Blm10 and human 20S proteasome activator 200 (PA200) are homologs. Comparative genome-wide analyses of untreated diploid cells lacking Blm10 and growing at steady state at defined growth rates revealed downregulation of numerous genes required for accurate chromosome structure, assembly and repair, and upregulation of a specific subset of genes encoding protein-folding chaperones.

Expression of the expanded YFL007w ORF and assignment of the gene name BLM10.

This report assigns a gene name to an extended ORF in Saccharomyces cerevisiae, and provides the first evidence from Northern analyses that the ORF is expressed and the transcript is the predicted size.