Structure-function correlation of localized visual field defects and macular microvascular damage in primary open-angle glaucoma.

Purpose: To investigate regional alterations of macular microvasculature in primary open-angle glaucoma (POAG), and to determine the structure-function correlation between localized visual field defects and macular microvascular damage in matching sectors.

Methods: Fifty-eight patients with POAG and 27 normal controls were recruited for this study. Optical coherence tomography angiography (OCTA) was used to measure retinal vascular microcirculation of the macula in superior, inferior, temporal, and nasal sectors.

Parapapillary Choroidal Microvasculature Dropout Is Associated With the Decrease in Retinal Nerve Fiber Layer Thickness: A Prospective Study.

Purpose: To explore the correlation between longitudinal changes of peripapillary retinal nerve fiber layer (RNFL) thickness and the presence of parapapillary choroidal microvasculature dropout (MvD).

Methods: This is a longitudinal cohort study. All patients with normal-tension glaucoma (NTG) were recruited from the Wenzhou Glaucoma Progression Study.

Thickness changes in the corneal epithelium and Bowman's layer after overnight wear of silicone hydrogel contact lenses.

Background: To investigate thickness changes in the corneal epithelium and Bowman's layer after overnight silicone hydrogel contact lens (CL) wear by using ultra-high resolution optical coherence tomography (UHROCT).

Methods: Eleven subjects without CL wearing history were recruited for this study. An UHROCT was used to measure the thickness of the epithelium (ET), Bowman's layer (BT), stroma (ST), and total cornea (CCT) at the center of both eyes.

Evaluation of Reliability and Validity of Three Common Dry Eye Questionnaires in Chinese.

Purpose: To investigate the psychometric properties of three commonly used dry eye questionnaires including McMonnies Questionnaire (MQ), the Ocular Surface Disease Index (OSDI), and the Salisbury Eye Evaluation Questionnaire (SEEQ) in Chinese.

Methods: This prospective cross-sectional study was conducted at the Eye Hospital of Wenzhou Medical University. Ninety-eight participants completed three questionnaires in a random order.

Long scan depth optical coherence tomography on imaging accommodation: impact of enhanced axial resolution, signal-to-noise ratio and speed.

Background: Spectral domain optical coherence tomography (SD-OCT) was a useful tool to study accommodation in human eye, but the maximum image depth is limited due to the decreased signal-to-noise ratio (SNR). In this study, improving optical resolutions, speeds and the SNR were achieved by custom built SD-OCT, and the evaluation of the impact of the improvement during accommodation was investigated.

Methods: Three systems with different spectrometer designs, including two Charge Coupled Device (CCD) cameras and one Complementary Metal-Oxide-Semiconductor Transistor (CMOS) camera, were tested.

Measurement variability of the bulbar conjunctival microvasculature in healthy subjects using functional slit lamp biomicroscopy (FSLB).

The goal was to determine the variability of the quantitative measurement of the bulbar conjunctival microvascular morphology and hemodynamics by testing the repeatability and variation during office hours. Functional slit-lamp biomicroscopy (FSLB) was used to image the bulbar conjunctival microvasculature, including the vessel diameter, blood flow velocity/rate and fractal dimensions of the microvascular network. The temporal side of the bulbar conjunctiva in 20 healthy subjects was imaged.

Age-related changes in the anterior segment biometry during accommodation.

Purpose: We investigated the dynamic response of human accommodative elements as a function of age during accommodation using synchronized spectral domain optical coherence tomography devices (SD-OCT).

Methods: We enrolled 33 left eyes from 33 healthy subjects (age range, 20-39 years, 17 males and 16 females). Two SD-OCT devices were synchronized to simultaneously image the anterior segment through pupil and the ciliary muscle during 6.

Vertical and horizontal corneal epithelial thickness profile using ultra-high resolution and long scan depth optical coherence tomography.

Purpose: To determine the vertical and horizontal thickness profiles of the corneal epithelium in vivo using ultra-long scan depth and ultra-high resolution spectral domain optical coherence tomography (SD-OCT).

Methods: A SD-OCT was developed with an axial resolution of ∼ 3.3 µm in tissue and an extended scan depth.

Whole eye axial biometry during accommodation using ultra-long scan depth optical coherence tomography.

Purpose: To investigate changes of whole eye axial biometry during accommodation using ultra-long scan depth optical coherence tomography (UL-OCT).

Design: Prospective, observational case series.

Methods: Twenty-one adult subjects were enrolled.

Functional slit lamp biomicroscopy for imaging bulbar conjunctival microvasculature in contact lens wearers.

Purpose: To develop, test and validate functional slit lamp biomicroscopy (FSLB) for generating non-invasive bulbar conjunctival microvascular perfusion maps (nMPMs) and assessing morphometry and hemodynamics.

Methods: FSLB was adapted from a traditional slit-lamp microscope by attaching a digital camera to image the bulbar conjunctiva to create nMPMs and measure venular blood flow hemodynamics. High definition images with a large field of view were obtained on the temporal bulbar conjunctiva for creating nMPMs.

Axial biometry of the entire eye using ultra-long scan depth optical coherence tomography.

Purpose: To assess the repeatability of axial biometry of the entire eye using ultra-long scan depth optical coherence tomography (OCT) and to investigate the agreement with IOLMaster measurements (Carl Zeiss Meditec).

Design: Prospective, observational case series.

Methods: There were 37 adult subjects enrolled in group 1 and 12 adult subjects enrolled in group 2.

Phacoemulsification induced transient swelling of corneal Descemet's Endothelium Complex imaged with ultra-high resolution optical coherence tomography.

Purpose: Thickness changes of corneal sub-layers after phacoemulsification were investigated by spectral domain ultra-high resolution optical coherence tomography (UHR-OCT).

Methods: The corneas (n = 26) of 26 age-related cataract surgery patients were studied. UHR-OCT was used to evaluate the thickness of Descemet's Endothelium Complex (DEC), stroma, Bowman's layer, epithelium, and full cornea at the center (CCT) before, one day after, and one week after surgery.

Ocular anterior segment biometry and high-order wavefront aberrations during accommodation.

Purpose: We investigated the relationships between the ocular anterior segment biometry and the ocular high-order aberrations (HOAs) during accommodation by combined ultralong scan depth optical coherence tomography (UL-OCT) and wavefront sensor.

Methods: We enrolled 35 right eyes of young healthy subjects (21 women and 14 men; age, 25.6 ± 3.

High resolution OCT quantitative analysis of the space between the IOL and the posterior capsule during the early cataract postoperative period.

Purpose: We quantitatively characterized the space between the IOL and the posterior capsule (IOL-PC space) during the early postphacoemulsification period, using high resolution optical coherence tomography (OCT).

Methods: We recruited 30 eyes of 30 patients who underwent phacoemulsification and randomly divided them into two groups. Acrysof Natural IQ IOLs were implanted in one group (n = 15), and Adapt-AO IOLs were implanted into the other (n = 15).

Entire thickness profiles of the epithelium and contact lens in vivo imaged with high-speed and high-resolution optical coherence tomography.

Purpose: To test the feasibility of measuring the entire thickness profiles of the epithelium and contact lens (CL) in vivo, using high-speed and high-resolution spectral-domain optical coherence tomography (SD-OCT).

Methods: A custom-built, long scan depth SD-OCT was developed based on a complementary metal oxide semiconductor (CMOS) camera, and the axial resolution was approximately 5.1 μm in tissue.

Ultra-high resolution and long scan depth optical coherence tomography with full-phase detection for imaging the ocular surface.

We used a unique combination of four state-of-the-art technologies to achieve a high performance spectral domain optical coherence tomography system suitable for imaging the entire ocular surface. An ultra-high resolution, extended depth range, full-phase interferometry, and high-speed complementary metal-oxide semiconductor transistor camera detection provided unprecedented performance for the precise quantification of a wide range of the ocular surface. We demonstrated the feasibility of this approach by obtaining high-speed and high-resolution images of a model eye beyond the corneal-scleral junction.

Versatile optical coherence tomography for imaging the human eye.

We demonstrated the feasibility of a CMOS-based spectral domain OCT (SD-OCT) for versatile ophthalmic applications of imaging the corneal epithelium, limbus, ocular surface, contact lens, crystalline lens, retina, and full eye in vivo. The system was based on a single spectrometer and an alternating reference arm with four mirrors. A galvanometer scanner was used to switch the reference beam among the four mirrors, depending on the imaging application.

Automated segmentation and fractal analysis of high-resolution non-invasive capillary perfusion maps of the human retina.

The retina provides a window to study the pathophysiology of cerebrovascular diseases. Pathological retinal microvascular changes may reflect microangiopathic processes in the brain. Recent advances in optical imaging techniques have enabled the imaging of the retinal microvasculature at the capillary level, and the generation of high-resolution, non-invasive capillary perfusion maps (nCPMs) with the Retinal Function Imager (RFI).

Resolution of slit-lamp microscopy photography using various cameras.

Purpose: To evaluate the resolutions of slit-lamp microscopy photography using various cameras.

Design: Evaluation of diagnostic test or technology.

Participants: Healthy subjects were imaged with these adapted cameras through slit-lamp microscopy.

The role of axial resolution of optical coherence tomography on the measurement of corneal and epithelial thicknesses.

Purpose: Our purpose was to investigate the role of the axial resolution of optical coherence tomography (OCT) on the measurement of corneal and epithelial thickness by evaluating the repeatability and agreement among different OCT devices with different axial resolutions.

Methods: twenty right eyes of 20 healthy subjects (AGE: 22.3 ± 1.

Human conjunctival microvasculature assessed with a retinal function imager (RFI).

The conjunctival and cerebral vasculatures share similar embryological origins, with similar structural and physiological characteristics. Tracking the conjunctival microvasculature may provide useful information for predicting the onset, progression and prognosis of both systemic and central nervous system (CNS) vascular diseases. The bulbar conjunctival vasculature was imaged using a retinal function imager (RFI, Optical Imaging Ltd, Rehovot, Israel).

Quantitative analysis of the intraretinal layers and optic nerve head using ultra-high resolution optical coherence tomography.

This study is designed to test the repeatability of the quantitative analysis of intraretinal layer thickness and cup-disc ratio of the optic nerve head using ultra-high resolution optical coherence tomography (UHR-OCT). Group A, containing 23 eyes of 12 healthy subjects, was imaged twice and group B, containing eight eyes of four subjects, was imaged three times. Intraretinal layers were segmented manually and the cup-to-disc ratio of the optic nerve head was analyzed.

Effect of punctal occlusion on tear menisci in symptomatic contact lens wearers.

Purpose: To investigate by ultrahigh resolution optical coherence tomography the effect of punctal occlusion on tear menisci in symptomatic and asymptomatic contact lens wearers.

Methods: Symptomatic subjects with self-reported dry eyes (n = 20) and asymptomatic subjects (n = 20) were recruited. For each subject, 1 eye was randomly chosen for both upper and lower punctal occlusion with collagen plugs.