Multispectral detection of dietary fiber content in Chinese cabbage leaves across different growth periods.

Multispectral imaging, combined with stoichiometric values, was used to construct a prediction model to measure changes in dietary fiber (DF) content in Chinese cabbage leaves across different growth periods. Based on all the spectral bands (365-970 nm) and characteristic spectral bands (430, 880, 590, 490, 690 nm), eight quantitative prediction models were established using four machine learning algorithms, namely random forest (RF), backpropagation neural network, radial basis function, and multiple linear regression. Finally, a quantitative prediction model of RF learning algorithm is constructed based on all spectral bands, which has good prediction accuracy and model robustness, prediction performance with R of 0.

Clinical nursing application of parenteral nutrition combined with enteral nutrition support in neurosurgery.

Background: To explore the clinical nursing effect of parenteral nutrition combined with enteral nutrition support in neurosurgery.

Methodology: 200 neurosurgical patients were randomly divided into two groups. The time of parenteral nutrition combined with enteral nutrition support in our hospital (January 2021) was used as the cut-off point, the PN group and the PN+EN group were divided according to the cut-off point.

Hormones and carbohydrates synergistically regulate the formation of swollen roots in a Chinese cabbage translocation line.

The genus contains a rich diversity of species and morphological types, including leaf, root, and oil crops, all of which show substantial phenotypic variation. Both Chinese cabbage and cabbage are typical leaf-type crops with normal roots. We created translocation lines based on interspecific crosses between Chinese cabbage and cabbage and identified qdh225, which exhibited a swollen-root phenotype.

Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop Improvement and Its Advancement towards Epigenetics.

Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technology that has evolved into an indispensable approach for analyzing the function of genes. It downregulates endogenous genes by utilizing the posttranscriptional gene silencing (PTGS) machinery of plants to prevent systemic viral infections. Based on recent advances, VIGS can now be used as a high-throughput tool that induces heritable epigenetic modifications in plants through the viral genome by transiently knocking down targeted gene expression.

Mutation in a chlorophyll-binding motif of Brassica ferrochelatase enhances both heme and chlorophyll biosynthesis.

The heme branch of tetrapyrrole biosynthesis contributes to the regulation of chlorophyll levels. However, the mechanism underlying the balance between chlorophyll and heme synthesis remains elusive. Here, we identify a dark green leaf mutant, dg, from an ethyl methanesulfonate (EMS)-induced mutant library of Chinese cabbage.

Genome-Wide Identification, Characterization, and Transcriptomic Analysis of the Cyclin Gene Family in .

Cyclins are involved in cell division and proliferation by activating enzymes required for the cell cycle progression. Our genome-wide analysis identified 76 cyclin genes in Brassica rapa, which were divided into nine different types (A-, B-, C-, D-, H-, L-, P-, T-, and SDS-type). Cyclin genes were unevenly scattered on all chromosomes, with a maximum of 10 on A08 and a minimum of 2 on A04.

Metabolomic and transcriptomic analyses identify quinic acid protecting eggplant from damage caused by western flower thrips.

Background: Western flower thrips are considered the major insect pest of horticultural crops worldwide, causing economic and yield loss to Solanaceae crops. The eggplant (Solanum melongena L.) resistance against thrips remains largely unexplored.

OCTOPUS regulates BIN2 to control leaf curvature in Chinese cabbage.

Heading is one of the most important agronomic traits for Chinese cabbage crops. During the heading stage, leaf axial growth is an essential process. In the past, most genes predicted to be involved in the heading process have been based on leaf development studies in .

Underlying Biochemical and Molecular Mechanisms for Seed Germination.

With the burgeoning population of the world, the successful germination of seeds to achieve maximum crop production is very important. Seed germination is a precise balance of phytohormones, light, and temperature that induces endosperm decay. Abscisic acid and gibberellins-mainly with auxins, ethylene, and jasmonic and salicylic acid through interdependent molecular pathways-lead to the rupture of the seed testa, after which the radicle protrudes out and the endosperm provides nutrients according to its growing energy demand.

Construction of a high-density mutant population of Chinese cabbage facilitates the genetic dissection of agronomic traits.

Chinese cabbage (Brassica rapa ssp. pekinensis) is an economically important vegetable crop throughout the world, especially in Asia. High-quality genome sequences are available for Chinese cabbage, but gene functional studies remain challenging.

Genetic analysis of the "head top shape" quality trait of Chinese cabbage and its association with rosette leaf variation.

The agricultural and consumer quality of Chinese cabbage is determined by its shape. The shape is defined by the folding of the heading leaves, which defines the head top shape (HTS). The overlapping HTS, in which the heading leaves curve inward and overlap at the top, is the shape preferred by consumers.

Development and Application of SSR Markers Related to Genes Involved in Leaf Adaxial-Abaxial Polarity Establishment in Chinese Cabbage ( L. ssp. ).

In Chinese cabbage ( L. ssp. ), leaf adaxial-abaxial (ad-ab) polarity is tightly related to leaf incurvature, an essential factor for the formation of leafy heads.

Gene characterization and molecular pathway analysis of reverse thermosensitive genic male sterility in eggplant ( L.).

The naturally occurring mutant eggplant line 05ms was identified with reverse thermosensitive genic male sterility (rTGMS), but its temperature-responsive fertility mechanisms remain largely unknown. Here, we studied the flower morphology, anther cellular structure, and genome-wide gene expression of this rTGMS line. Candidate genes for thermosensitive male sterility during the microspore development of 05ms and the temperature-insensitive line S63 under low-temperature (LT) and high-temperature (HT) conditions were identified.

Comparative transcriptome analysis reveals defense responses against soft rot in Chinese cabbage.

ssp. () is a necrotrophic bacterial species that causes soft rot disease in Chinese cabbage. In this study, plants harboring the resistant mutant gene, which confers resistance against , were screened from an 800 M population mutated by ethyl methane sulfonate (EMS) and scored in vitro and in vivo for lesion size.

Characterization of Non-heading Mutation in Heading Chinese Cabbage ( L. ssp. ).

Heading is a key agronomic trait of Chinese cabbage. A non-heading mutant with flat growth of heading leaves () was isolated from an EMS-induced mutant population of the heading Chinese cabbage inbred line A03. In mutant plants, the heading leaves are flat similar to rosette leaves.

Coupling Seq-BSA and RNA-Seq Analyses Reveal the Molecular Pathway and Genes Associated with Heading Type in Chinese Cabbage.

In Chinese cabbage, heading type is a key agricultural trait of significant economic importance. Using a natural microspore-derived doubled haploid plant, we generated self-crossed progeny with overlapping or outward curling head morphotypes. Sequencing-based bulked segregant analysis (Seq-BSA) revealed a candidate region of 0.

Microspore Induced Doubled Haploids Production from Ethyl Methanesulfonate (EMS) Soaked Flower Buds Is an Efficient Strategy for Mutagenesis in Chinese Cabbage.

Chinese cabbage buds were soaked with Ethyl methanesulfonate (EMS) to induce mutagenesis. The influence of different EMS concentrations and treatment durations on microspore development, embryo production rate and seedling rate were evaluated in five Chinese cabbage genotypes. Mutations in four color-related genes were identified using high resolution melting (HRM) curves of their PCR products.

Porous Cu-NiO modified glass carbon electrode enhanced nonenzymatic glucose electrochemical sensors.

Porous Cu-NiO nanocomposites were successfully prepared by calcination of the Cu-Ni(OH)(2) precursor at 400 °C for 2 h. During the process of calcination, Ar was used to deaerate O(2). The structure and morphology of Cu-NiO were characterized by X-ray diffraction spectrum (XRD), energy dispersive X-ray analyses (EDX), transmission electron microscopy (TEM), and scanning electron microscopy (SEM).

Silver oxide nanowalls grown on Cu substrate as an enzymeless glucose sensor.

Ag(2)O nanowalls consisting of densely packed nanoplates based on a Cu substrate were synthesized through a facile one-pot hydrothermal method. A new enzymeless glucose sensor of Cu-Ag(2)O nanowalls was fabricated. The Cu-Ag(2)O nanowalls showed higher catalysis on glucose oxidation than traditional Ag(2)O nanoflowers and Cu-Ag(2)O nanospindles.

CuS nanotubes for ultrasensitive nonenzymatic glucose sensors.

CuS nanotubes made up of nanoparticles were successfully prepared in large quantities in an O/W microemulsion system under low temperature; the as-prepared CuS nanotube modified electrode was used as an enzyme-free glucose sensor.