Application of Response Surface Methodology for Optimization of Extracellular Glucoamylase Production by Candida guilliermondii.

Objective: Glucoamylase is among the most important enzymes in biotechnology. The present study aims to determine better conditions for growth and glucoamylase production by Candida guilliermondii and to reduce the overall cost of the medium using Box-Behnken design with one central point and response surface methodology.

Methodology: Box-Behnken factorial design based on three levels was carried out to obtain optimal medium combination of five independent variables such as initial pH, soluble starch, CH4N2O, yeast extract and MgSO4.

Optimization of composition of media for the production of extracellular glucoamylase by Candida guilliermendii.

The combined effects of macronutrients of media on glucoamylase production by Candida guilliermendii were studied using Design Of Experiment (DOE). A 2(P-k) factorial design was chosen to explain fifteen medium constituents: pH, Starch, Sucrose, Yeast extract, Peptone, NH4Cl (NH4)2HPO4, NH4NO3, CH4NO2 (NH4)2SO4, CaCl2, MnCl2, FeCl2, ZnCl2 and MgCl2 and analyse the results. This procedure limited the number of actual experiments performed while allowing for possible interactions between components.