Antioxidant activity assays on-line with liquid chromatography.

Screening for antioxidants requires simple in vitro model systems to investigate antioxidant activity. High resolution screening (HRS), combining a separation technique like HPLC with fast post-column (bio)chemical detection can rapidly pinpoint active compounds in complex mixtures. In this paper both electrochemical and chemistry-based assays are reviewed and discussed.

Improving the in vivo predictability of an on-line HPLC stable free radical decoloration assay for antioxidant activity in methanol-buffer medium.

The pivotal role of ROS (reactive oxygen species) in various (patho)physiological processes has stimulated research on the potential of intervening in these processes with antioxidants (AO). In vitro model systems to investigate AO activity against the various ROS are a valuable tool in classifying antioxidants. To improve the in vivo predictability of the results obtained, we have modified and characterized the widely used DPPH (2,2'-diphenyl-1-picrylhydrazyl) on-line decoloration assay.