The consecutive rollout of mRNA vaccines has sparked discussions about their long-term safety, expression, and potential for integrating into the genome.
In this study, researchers evaluated the longevity of antigenic protein expression from self-amplifying mRNA (SAM) vaccines in different cell lines, confirming high levels of protein expression and stability.
The results showed no genomic integration of the mRNA and produced promising 3D structures of associated proteins, indicating the potential of the alphavirus-based SAM platform for future vaccine development.