Automatic high-throughput and non-invasive selection of sperm at the biochemical level.

Background: Sperm selection, a key step in assisted reproductive technology (ART), has long been restrained at the preliminary physical level (morphology or motility); however, subsequent fertilization and embryogenesis are complicated biochemical processes. Such an enormous "gap" poses tough problems for couples dealing with infertility, especially patients with severe/total asthenozoospermia .

Methods: We developed a biochemical-level, automatic-screening/separation, smart droplet-TO-hydrogel chip (BLASTO-chip) for sperm selection.

Inferring Causal Relationships Between Metabolites and Polycystic Ovary Syndrome Using Summary Statistics from Genome‑Wide Association Studies.

Polycystic ovary syndrome (PCOS) is a disorder characterized by hyperandrogenism, ovulatory dysfunction, and polycystic ovarian morphology. Previous studies have suggested that metabolites may play a pivotal mediating role in the progression of phenotypic variations. Although several metabolites had been identified as potential markers for PCOS, the relationship between blood metabolites and PCOS was not comprehensively explored.

A de novo mutation of ADAMTS8 in a patient with Wiedemann-Steiner syndrome.

Background: Wiedemann-Steiner syndrome (WDSTS) is a rare autosomal dominant disorder caused by mutations in the KMT2A gene and is usually characterized by hairy elbows, short stature, developmental delay, intellectual disability and obvious facial dysmorphism.

Case Presentation: Here, we report a 5-year-old girl with clinical features similar to WDSTS, including postnatal growth delay, retarded intellectual development, and ocular hypertelorism. Through whole-exome sequencing (WES), a frameshift variant of KMT2A was found in the patient but not in her parents' genomic DNA.

Functional Enrichment Analysis Identifying Regulatory Information Associated with Human Fracture.

Dozens of loci associated with fracture have been identified by genome-wide association studies (GWASs). However, most of these variants are located in the noncoding regions including introns, long terminal repeats, and intergenic regions. Although combining regulation information helps to identify the causal SNPs and interpret the involvement of these variants in the etiology of human fracture, regulation information which was truly associated with fracture was unknown.

Dramatic improvement in the mechanical properties of polydopamine/polyacrylamide hydrogel mediated human amniotic membrane.

Human amniotic membrane (hAM) is a promising material for tissue engineering due to several benefits, including desirable biocompatibility, stem cell source, antibacterial activity, . However, because of its low elasticity, the clinical application of hAM is severely restricted. To solve this issue, we employed polydopamine/polyacrylamide (PDA/PAM) hydrogels to toughen hAM.

Stigmatization and Interpersonal Deviance Behaviors of Tour Guides: The Influence of Self-Identity Threat and Moral Disengagement.

Severe tour guide stigma is a significant problem hindering tourism development. Based on self-identity threat and moral disengagement theory, this study analyzed the relationship between tour guide stigmatization and tour guides' interpersonal deviance behavior. Survey data collected from 241 tour guides at three different points in time showed that tour guide stigmatization was positively related to tour guides' interpersonal deviance behavior and that self-identity threat mediated this effect.

Identification of Multiple Cracks in Composite Laminated Beams Using Perturbation to Dynamic Equilibrium.

Identification of cracks in beam-type components is significant to ensure the safety of structures. Among the approaches relying on mode shapes, the concept of transverse pseudo-force (TPF) has been well proved for single and multiple crack identification in beams made of isotropic materials; however, there is a noticeable gap between the concept of TPF and its applications in composite laminated beams. To fill this gap, an enhanced TPF approach that relies on perturbation to dynamic equilibrium is proposed for the identification of multiple cracks in composite laminated beams.

The Use of CRISPR/Cas9 as a Tool to Study Human Infectious Viruses.

Clustered regularly interspaced short palindromic repeats (CRISPR) systems are a set of versatile gene-editing toolkit that perform diverse revolutionary functions in various fields of application such as agricultural practices, food industry, biotechnology, biomedicine, and clinical research. Specially, as a novel antiviral method of choice, CRISPR/Cas9 system has been extensively and effectively exploited to fight against human infectious viruses. Infectious diseases including human immunodeficiency virus (HIV), hepatitis B virus (HBV), human papillomavirus (HPV), and other viruses are still global threats with persistent potential to probably cause pandemics.

Measurement and Spatial Variation of Green Total Factor Productivity of the Tourism Industry in China.

Promoting tourism in China using sustainable practices has become a very important issue. In order to analyze temporal characteristics and spatial regularities of green total factor productivity (GTFP), carbon emissions and the consumption of energy related to tourism in China were estimated using a "bottom-up" method. The construction of a measurement framework (including carbon emissions and energy consumption) of GTFP for the tourism industry was also undertaken.

Assessment of aneuploidy formation in human blastocysts resulting from cryopreserved donor eggs.

Background: Increased embryo implantation rates were reported after transfer of euploid embryos selected by preimplantation genetic screening (PGS). Egg cryopreservation by vitrification has become one of the most important assisted human reproduction technologies. Although reports indicate that development and implantation of human embryos derived from frozen donor eggs are comparative to fresh eggs, it is still unknown whether egg vitrification increases chromosomal abnormalities in eggs, which in turn causes formation of embryonic aneuploidy.

A solvothermal method to synthesize fluorescent carbon nanoparticles and application to photocatalysis and electrocatalysis.

A novel solvothermal approach to synthesize fluorescent carbon nanoparticles (CNPs) was developed using glucose and ammonium oxalate as the carbon source, and glycol as the solvent. The solution of as-prepared CNPs emitted blue-green fluorescence under ultraviolet (UV) light at 365 nm, and the carbon nanoparticle formation was investigated through XRD, TEM, DLS, FT-IR, UV, PL, XPS. The particle was well dispersed with an average diameter of about 10-30 nm.

Multipoint subcutaneous injection of long-acting glucocorticid as a cure for pretibial myxedema.

Background: Pretibial myxedema (PM) is an uncommon and characteristic manifestation of Graves' disease (GD), with local autoimmune reaction in cutaneous tissue. The treatment of PM is a clinical challenge. We herein report a patient with PM who achieved complete remission by multipoint subcutaneous injection of long-acting glucocorticid.