Effect and mechanisms of thermal sterilization methods on the in vitro phenolic bioaccessibility of rose tea with milk.

Rose polyphenols, key functional components in roses, require adequate bioaccessibility for their health benefits, subject to influence by food components and processing. Investigating the impact of various thermal sterilization methods on the bioaccessibility of rose polyphenols in rose tea with milk and the underlying mechanisms, our findings indicated a significant increase in bioaccessibility following treatment at 85 °C/30 min. Conversely, 121 °C/15 min treatment decreased bioaccessibility.

A Ni-NTA-based red fluorescence probe for protein labelling in live cells.

The great success of a His-Ni-nitrilotriaceate (Ni-NTA) system in protein purification has inspired scientists to develop novel Ni-NTA based fluoresent probes for imaging of proteins in live cells. Despite a great deal of effort being made, only very few such probes can cross the cell membrane to label intracellular proteins. Herein, a new red fluorescence probe (Ni-NTA-AB) consisting of a Ni-NTA moiety, BODIPY and an arylazide was designed and synthesized to selectively label His-tagged proteins.

Rapid labeling of intracellular His-tagged proteins in living cells.

Small molecule-based fluorescent probes have been used for real-time visualization of live cells and tracking of various cellular events with minimal perturbation on the cells being investigated. Given the wide utility of the (histidine)6-Ni(2+)-nitrilotriacetate (Ni-NTA) system in protein purification, there is significant interest in fluorescent Ni(2+)-NTA-based probes. Unfortunately, previous Ni-NTA-based probes suffer from poor membrane permeability and cannot label intracellular proteins.