Fluorogenic Crystallization via Enzyme-Instructed Excited-State Intramolecular Proton Transfer for Dynamic Microenvironment Profiling.

Understanding dynamic changes within cellular microenvironments is crucial for elucidating biological processes and developing targeted therapies. Here, we present a rapid and straightforward strategy for profiling tumor microenvironments via fluorogenic crystallization driven by enzyme-instructed excited-state intramolecular proton transfer (ESIPT). By engineering ESIPT-based fluorophores, we achieve selective crystallization with strong dual-emission ratiometric fluorescence signals that are easily visualized, offering a real-time readout of tumor microenvironmental variations.

Mechanism of two styryl BODIPYs as fluorescent probes and protective agents in lipid bilayers against aqueous ClO.

Two styryl BODIPY derivatives, BOH and BOE, with different hydrophilic properties, were investigated for their reaction mechanisms in lipid bilayers against aqueous ClO, by both experimental and theoretical methods. Density functional theory (DFT) calculations confirmed their identical conformations in solution. Fluorescence spectra and high-resolution mass spectra corroborated the central vinyl group as a common antioxidation moiety against ClO oxidation.

What Are the Reliable Plasma Biomarkers for Mild Cognitive Impairment? A Clinical 4D Proteomics Study and Validation.

Objective: At present, Alzheimer's disease (AD) lacks effective treatment means, and early diagnosis and intervention are the keys to treatment. Therefore, for mild cognitive impairment (MCI) and AD patients, blood sample analysis using the 4D nonstandard (label-free) proteomic in-depth quantitative analysis, looking for specific protein marker expression differences, is important. These marker levels change as AD progresses, and the analysis of these biomarkers changes with this method, which has the potential to show the degree of disease progression and can be used for the diagnosis and preventive treatment of MCI and AD.