Publications by authors named "Ailiang Jiang"

() is a traditional edible and medicinal mushroom in China. The main bioactive components in include triterpenoids, polysaccharides, steroids, and sterols. Ganoderic acids (GAs) are one of the most abundant triterpenoids found in , garnering significant attention from researchers in the fields of medicine and health care.

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In food industry, the characteristics of food substrate could be improved through its bidirectional solid-state fermentation (BSF) by fungi, because the functional components were produced during BSF. Six edible fungi were selected for BSF to study their effects on highland barley properties, such as functional components, antioxidant activity, and texture characteristics. After BSF, the triterpenes content in Ganoderma lucidum and Ganoderma leucocontextum samples increased by 76.

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, the most produced edible mushroom species in China, is rich in lysine. Further enhancing its lysine biosynthesis is vital for improving its quality in industrialized cultivation. Citric acid induction significantly increases both the biomass and growth rate of hyphae, as well as the lysine content.

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Carbon monoxide (CO), a product of organic oxidation processes, arises in vivo principally from the enzymatic reaction of heme oxygenase (HO, transcription gene named ). HO/CO has been found to exert many salutary effects in multiple biological processes, including the stress response. However, whether HO/CO is involved in the regulation of the heat-stress (HS) response of () is still poorly understood.

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Water stress affects both the growth and development of filamentous fungi; however, the mechanisms underlying their response to water stress remain unclear. In this study, water stress was found to increase intracellular reactive oxygen species (ROS) level, ganoderic acid (GA) content, and NADPH oxidase (NOX) activity of Ganoderma lucidum by 148.45%, 75.

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Putrescine (Put) has been shown to play an important regulatory role in cell growth in organisms. As the primary center regulating the homeostasis of polyamine (PA) content, ornithine decarboxylase antizyme (AZ) can regulate PA content through feedback. Nevertheless, the regulatory mechanism of Put is poorly understood in fungi.

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Article Synopsis
  • The text discusses the use of CRISPR/Cas9 technology for precision genome editing of a commercially important edible fungus, previously referred to as "Asian."
  • The authors successfully developed a genome-editing system using plasmids containing codon-optimized Cas9 and dual sgRNAs, resulting in mutants that show resistance to 5-fluorooric acid (5-FOA).
  • The study identified a native U6 promoter and demonstrated heritable genomic changes in the mutants, marking the first successful CRISPR/Cas9 application in this type of fungus, thus expanding its potential for molecular breeding.
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  • - The study aimed to enhance lysine content (a vital quality indicator) using chitosan, determining optimal conditions of 14.61 μg/mL concentration and 52.90 hours treatment, resulting in a theoretical lysine value of 30.95 mg/g.
  • - Genetic analysis identified 11 members of amino acid transporter (AAT) genes, with AAT3 and AAT4 showing significant expression increases after chitosan treatment and impacting lysine biosynthesis regulation.
  • - Chitosan treatment led to a 26.41% increase in lysine in the wild-type strain, while silencing AAT3 and AAT4 genes caused a decrease in lysine content, suggesting AAT3
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  • The study investigates the effects of the plant hormone methyl jasmonate (MeJA) on Ganoderma lucidum, a medicinal mushroom known for its active compounds, ganoderic acids (GAs).
  • Researchers utilized integrated proteomics and metabolomics to identify differences in proteins and metabolites after 15 minutes and 24 hours of MeJA treatment, uncovering 209 differential abundance proteins (DAPs) and 154 metabolites linked to various metabolic pathways.
  • The findings suggest that MeJA induces a metabolic shift that inhibits normal cellular processes like glucose metabolism while enhancing the production of secondary metabolites, particularly GAs, providing insights for future research on MeJA signaling and GA biosynthesis.
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Article Synopsis
  • Fungi often produce secondary metabolites like ganoderic acid (GA) at low levels, with regulatory mechanisms not well understood until recently.
  • Ganoderma lucidum, a traditional Chinese medicinal mushroom, has GA as a key ingredient, and environmental factors such as chemical signals, physical triggers, and nutritional conditions influence its biosynthesis.
  • Understanding the regulatory processes in G. lucidum, involving pathways like ROS, Ca, and cAMP signaling, can help researchers study secondary metabolism in other fungi, especially those that are harder to manipulate genetically.
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Isoforms of 14-3-3 proteins, similar to their highly conserved homologs in mammals and plants, are both transcriptionally and functionally affected by their extracellular and intracellular environments. These proteins bind to phosphorylated client proteins to modulate their functions in fungi. Since phosphorylation regulates a plethora of different physiological responses in organisms, 14-3-3 proteins play roles in multiple physiological functions, including those controlling metabolisms, cell division, and responses to environmental stimulation.

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How cells drive the phospholipid signal response to heat stress (HS) to maintain cellular homeostasis is a fundamental issue in biology, but the regulatory mechanism of this fundamental process is unclear. Previous quantitative analyses of lipids showed that phosphatidylinositol (PI) accumulates after HS in Ganoderma lucidum, implying the inositol phospholipid signal may be associated with HS signal transduction. Here, we found that the PI-4-kinase and PI-4-phosphate-5-kinase activities are activated and that their lipid products PI-4-phosphate and PI-4,5-bisphosphate are increased under HS.

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is among the best known medicinal basidiomycetes due to its production of many pharmacologically active compounds. To study the regulatory networks involved in its growth and development, we analyzed the relationship between reactive oxygen species (ROS) and Ca signaling in the regulation of hyphal branching and ganoderic acid (GA) biosynthesis after Cu treatment. Our results revealed that Cu treatment decreased the distance between hyphal branches and increased the GA content and the intracellular levels of ROS and Ca Further research revealed that the Cu-induced changes in hyphal branch distance, GA content, and cytosolic Ca level were dependent on increases in cytosolic ROS.

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Ganoderma lucidum has high commercial value because it produces many active compounds, such as ganoderic acids (GAs). Salicylic acid (SA) was previously reported to induce the biosynthesis of GA in G. lucidum.

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The APSES transcription factors have been identified as key regulators of fungal development and other biological processes in fungi. In the present study, the function of Ganoderma lucidum GlSwi6, a homolog of Saccharomyces cerevisiae Swi6, was characterized. RNAi was used to examine the function of GlSwi6 in G.

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Ganoderma lucidum has been considered an emerging model species for studying how environmental factors regulate the growth, development, and secondary metabolism of Basidiomycetes. Heat stress, which is one of the most important environmental abiotic stresses, seriously affects the growth, development, and yield of microorganisms. Understanding the response to heat stress has gradually become a hotspot in microorganism research.

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Phospholipid-mediated signal transduction plays a key role in responses to environmental changes, but little is known about the role of phospholipid signalling in microorganisms. Heat stress (HS) is one of the most important environmental factors. Our previous study found that HS could induce the biosynthesis of the secondary metabolites, ganoderic acids (GA).

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We demonstrate herein that salicylic acid (SA) can enhance ganoderic acid (GA) accumulation in the lingzhi or reishi medicinal mushroom Ganoderma lucidum. Following treatment with different concentrations of SA, the GA content was increased 22.72% to 43.

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Ganoderma lucidum has become a potential model system for evaluating how environmental factors regulate the secondary metabolism of basidiomycetes. Heat stress (HS) is one of the most important environmental factors. It was previously reported that HS could induce the biosynthesis of ganoderic acids (GA).

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Ganoderma lucidum is a medicinal macrofungus that is widely used in traditional Chinese medicine. Nonetheless, the scarcity of basic biological studies of this organism has hindered the further development of its commercial value. The pH-responsive transcription factor PacC/Rim101 governs the adaptation to environmental pH, the development and the secondary metabolism of many fungi.

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Ganoderma lucidum is one of the most important medicinal fungi, but the lack of basic study on the fungus has hindered the further development of its value. To investigate the roles of the redox system in G. lucidum, acetic acid (HAc) was applied as a reactive oxygen species (ROS) stress inducer, and hydrogen-rich water (HRW) was used to relieve the ROS stress in this study.

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Unlabelled: Heat stress (HS) influences the growth and development of organisms. Thus, a comprehensive understanding of how organisms sense HS and respond to it is required. Ganoderma lucidum, a higher basidiomycete with bioactive secondary metabolites, has become a potential model system due to the complete sequencing of its genome, transgenic systems, and reliable reverse genetic tools.

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UDP-glucose pyrophosphorylase (UGP) is a key enzyme involved in carbohydrate metabolism, but there are few studies on the functions of this enzyme in fungi. The ugp gene of Ganoderma lucidum was cloned, and enzyme kinetic parameters of the UGP recombinant protein were determined in vitro, revealing that this protein was functional and catalyzed the reversible conversion between Glc-1-P and UDP-Glc. ugp silencing by RNA interference resulted in changes in the levels of the intermediate metabolites Glc-1-P and UDP-Glc.

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Ganoderma lucidum is a mushroom with traditional medicinal properties that has been widely used in China and other countries in Eastern Asia. Ganoderic acids (GA) produced by G. lucidum exhibit important pharmacological activities.

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A hydroxymethylglutaryl-CoA synthase gene, designated as GlHMGS (GenBank accession No. JN391469) involved in ganoderic acid (GA) biosynthesis pathway was cloned from Ganoderma lucidum. The full-length cDNA of GlHMGS (GenBank accession No.

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