Feasibility study of direct CT lymphangiography in mice: comparison with interstitial CT/MR lymphangiography.

Objectives: To establish a CT lymphangiography method in mice via direct lymph node puncture.

Methods: We injected healthy mice (n = 8) with 50 µl of water-soluble iodine contrast agent (iomeprol; iodine concentration, 350 mg/mL) subcutaneously into the left-rear foot pad (interstitial injection) and 20 µl of the same contrast agent directly into the popliteal lymph node (direct puncture) 2 days later. Additionally, we performed interstitial MR lymphangiography on eight mice as a control group.

Invariant NKT cells promote skin wound healing by preventing a prolonged neutrophilic inflammatory response.

The wound-healing process consists of the inflammation, proliferation, and remodeling phases. In chronic wounds, the inflammation phase is prolonged with persistent neutrophil infiltration. The inflammatory response is critically regulated by cytokines and chemokines that are secreted from various immune cells.

Contribution of CARD9-mediated signalling to wound healing in skin.

The inflammatory response after skin injury involves the secretion of a variety of cytokines and growth factors that are necessary for tissue repair. Caspase recruitment domain-containing protein 9 (CARD9) is an essential signalling adaptor molecule for NF-κB activation upon triggering through C-type lectin receptors (CLRs), which are expressed in macrophages and dendritic cells. However, the role of CARD9 in inflammatory responses at the wound site has not been elucidated.

Promotion of acute-phase skin wound healing by Pseudomonas aeruginosa C -HSL.

A Pseudomonas aeruginosa quorum-sensing system, which produces N-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-C -HSL) and N-butanoyl-l-homoserine lactone (C -HSL), regulates the virulence factors. In our previous study, 3-oxo-C -HSL, encoded by lasI gene, was shown to promote wound healing. However, the effect of C -HSL, encoded by rhlI gene, remains to be elucidated.

Contribution of Invariant Natural Killer T Cells to Skin Wound Healing.

In the present study, we determined the contribution of invariant natural killer T (iNKT) cells to the skin wound healing process. In iNKT cell-deficient (Jα18KO) mice lacking iNKT cells, wound closure was significantly delayed compared with wild-type mice. Collagen deposition, expression of α-smooth muscle actin and CD31, and wound breaking strength were significantly attenuated in Jα18KO mice.

Multimodal biopanning of T7 phage-displayed peptides reveals angiomotin as a potential receptor of the anti-angiogenic macrolide Roxithromycin.

Roxithromycin (RXM) is a semi-synthetic fourteen-membered macrolide antibiotic that shows anti-angiogenic activity in solid tumors. In the present study, we conducted biopanning of T7 phage-displayed peptides either on a 96-well formatted microplate, a flow injection-type quartz-crystal microbalance (QCM) biosensor, or a cuvette-type QCM. RXM-selected peptides of different sequence, length and number were obtained from each mode of screening.

A new phenolic glycoside syringate from the bark of Juglans mandshurica MAXIM. var. sieboldiana MAKINO.

A new phenolic glycoside syringate, 4'-hydroxy-2',6'-dimethoxyphenol 1-O-beta-D: -(6-O-syringoyl) glucopyranoside (1), together with two known ones, 2'-hydroxy-4'-methoxyphenol 1-O-beta-D: -(6-O-syringoyl) glucopyranoside (2) and 4'-hydroxy-2'-methoxyphenol 1-O-beta-D: -(6-O-syringoyl) glucopyranoside (3), were isolated from the bark of Juglans mandshurica MAXIM. var. sieboldiana MAKINO.