Yeast Surface-Displayed Quenchbody as a Novel Whole-Cell Biosensor for One-Step Detection of Influenza A (H1N1) Virus.

Timely surveillance of airborne pathogens is essential to preventing the spread of infectious diseases and safeguard human health. Methods for sensitive, efficient, and cost-effective detection of airborne viruses are needed. With advances in synthetic biology, whole-cell biosensors have emerged as promising platforms for environmental monitoring and medical diagnostics.

Building Environmental and Sociological Predictive Intelligence to Understand the Seasonal Threat of SARS-CoV-2 in Human Populations.

Current modeling practices for environmental and sociological modulated infectious diseases remain inadequate to forecast the risk of outbreak(s) in human populations, partly due to a lack of integration of disciplinary knowledge, limited availability of disease surveillance datasets, and overreliance on compartmental epidemiological modeling methods. Harvesting data knowledge from virus transmission (aerosols) and detection (wastewater) of SARS-CoV-2, a heuristic score-based environmental predictive intelligence system was developed that calculates the risk of COVID-19 in the human population. Seasonal validation of the algorithm was uniquely associated with wastewater surveillance of the virus, providing a lead time of 7-14 days before a county-level outbreak.

Improved performance of nucleic acid-based assays for genetically diverse norovirus surveillance.

Nucleic acid-based assays, such as polymerase chain reaction (PCR), that amplify and detect organism-specific genome sequences are a standard method for infectious disease surveillance. However, challenges arise for virus surveillance because of their genetic diversity. Here, we calculated the variability of nucleotides within the genomes of 10 human viral species and found that endemic viruses exhibit a high percentage of variable nucleotides (e.

Application of neighborhood-scale wastewater-based epidemiology in low COVID-19 incidence situations.

Wastewater-based epidemiology (WBE), an emerging approach for community-wide COVID-19 surveillance, was primarily characterized at large sewersheds such as wastewater treatment plants serving a large population. Although informed public health measures can be better implemented for a small population, WBE for neighborhood-scale sewersheds is less studied and not fully understood. This study applied WBE to seven neighborhood-scale sewersheds (average population of 1471) from January to November 2021.

Design of SARS-CoV-2 Variant-Specific PCR Assays Considering Regional and Temporal Characteristics.

Monitoring the prevalence of SARS-CoV-2 variants is necessary to make informed public health decisions during the COVID-19 pandemic. PCR assays have received global attention, facilitating a rapid understanding of variant dynamics because they are more accessible and scalable than genome sequencing. However, as PCR assays target only a few mutations, their accuracy could be reduced when these mutations are not exclusive to the target variants.

Protective Effects of Gallic Acid Against NiSO4-Induced Toxicity Through Down-Regulation of the Ras/ERK Signaling Pathway in Beas-2B Cells.

BACKGROUND This study aimed to explore the preventive effects of gallic acid (GA) on the toxicity induced by NiSO4 in Beas-2B cells. MATERIAL AND METHODS Beas-2B cell viability was measured by MTT assay. The degree of oxidative stress was detected by measuring the levels of reactive oxygen species (ROS) and lipid peroxide (LPO).

[Sediment and pore water nutrient characteristics in growing zones of Alternanthera philoxeroides in the Shiwuli River, Chaohu Lake].

In order to investigate the nitrogen and phosphorus characteristics of sediments and pore water in growing zones of aquatic macrophytes (Alternanthera philoxeroides), three classic representative sampling points in the planted Alternanthera philoxeroides fields (i. e. SP1, SP2 and SP3) and one reference site in the seldom vegetation (i.

[Distribution and bioavailability of nitrogen and phosphorus species in the urban dusts from Hefei City].

To find out the distribution and bioavailability of nitrogen (N) and phosphorus (P) species in the urban dusts of Hefei City, 52 samples were collected from impervious areas with six different urban land-use types. The contents of ammonia nitrogen (NH4(+) -N), nitrate nitrogen (NO3(-) -N), exchangeable P (Ex-P), Al-bound P (Al-P), Fe-bound P (Fe-P), occluded P (Oc-P), Ca-bound P (Ca-P), detrital apatite P (De-P), organic P (Or-P) as well as total nitrogen (TN) and total phosphorus (TP) were measured by sequential extraction methods. The studies on spatial distribution, correlation and bioavailability of nitrogen and phosphorus species were made according to the analyzed data.

[Distribution of metals in urban dusts of Hefei and health risk assessment].

This study focused on the characterization and the health risk assessment of heavy metals in the dust of Hefei City, China. Samples were collected from fifty two sampling points covering six land-use types. Most of the sites were impervious ground such as residential, commercial, industrial, educational and traffic areas, as well as public landscapes and city squares.