Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation.

A human induced pluripotent stem cell (iPSC) line was generated from patient with type 1 neurofibromatosis (NF-1), carrying heterozygous mutation in NF1 gene. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating delivery of KFL4, OCT4, SOX2, BCL-XL and c-MYC. The iPSC line expresses pluripotency markers, displays a normal karyotype, and is able to differentiate into three germ layers in vitro.

Establishment of a human induced pluripotent stem cell line (SDQLCHi079-A) from a patient with Johanson-Blizzard syndrome carrying heterozygous mutation in UBR1 gene.

Johanson-Blizzard syndrome (JBS) is an autosomal recessive disorder. We established an induced pluripotent stem cell (iPSC) line from peripheral blood mononuclear cells of a 2-year-old boy with Johanson-Blizzard syndrome carrying a compound heterozygous mutation of c.3167C>G (p.

Prediction of new cerebral ischemic lesion after carotid artery stenting: a high-resolution vessel wall MRI-based radiomics analysis.

Objectives: Carotid artery stenting (CAS) is an established treatment for local stenosis. The most common complication is new ipsilateral ischemic lesions (NIILs). This study aimed to develop models considering lesion morphological and compositional features, and radiomics to predict NIILs.

LncRNA HCG18 facilitates melanoma progression by modulating miR-324-5p/CDK16 axis.

Objective: LncRNA HCG18 has been reported to act as a tumor promoter in gastric cancer, hepatocellular carcinoma and nasopharyngeal carcinoma. However, the role of HCG18 in melanoma is still not clear. In this study, we detected the expression and molecular function of HCG18 in melanoma.

WDR5 promotes the proliferation of lung adenocarcinoma by inducing SOX9 expression.

WDR5 is a coactivator of transcription factor which promotes the progression of several cancer types, but its function in lung adenocarcinoma (AC) is unknown. We detected WDR5 expression in lung AC with quantitative real-time polymerase chain reaction and immunohistochemistry. WDR5 was significantly overexpressed in ACs compared with normal lung tissues.

Circumferential degree of carotid calcification is associated with new ischemic brain lesions after carotid artery stenting.

Background: The relationship between plaque calcification and new ischemic brain lesions after carotid artery stenting (CAS) remains controversial. The purpose of this study was to determine if the circumferential degree of carotid calcification is associated with new ischemic brain lesions on diffusion-weighted imaging (DWI) after CAS.

Methods: A total of 96 patients with carotid stenosis of ≥50% who underwent CAS were enrolled in the study.

Identification of high-risk carotid plaque with MRI-based radiomics and machine learning.

Objectives: We sought to build a high-risk plaque MRI-based model (HRPMM) using radiomics features and machine learning for differentiating symptomatic from asymptomatic carotid plaques.

Materials And Methods: One hundred sixty-two patients with carotid stenosis were randomly divided into training and test cohorts. Multi-contrast MRI including time of flight (TOF), T1- and T2-weighted imaging, and contrast-enhanced imaging was done.

Associations between carotid intraplaque hemorrhage and new ipsilateral ischemic lesions after carotid artery stenting: a quantitative study with conventional multi-contrast MRI.

The risk of cerebral embolism after CAS in patients with carotid IPH is still controversial. This study was to further investigate the relationship between IPH and new ipsilateral ischemic lesion (NIIL) after CAS, and to perform a volumetric analysis of IPH for predicting the risk of NIIL following CAS. One hundred and seventeen patients with carotid stenosis undergoing CAS were prospectively enrolled.

Heat shock protein-guided dual-mode CT/MR imaging of orthotopic hepatocellular carcinoma tumor.

The development of an ultrasensitive nanoprobe is regarded as one of the most promising strategies for the precise diagnosis of tumors. Herein, tanespimycin (17-AAG)-modified polyethylenimine-based gold nanoparticles (AuNPs) co-labeled with a gadolinium (Gd) nanoprobe are constructed for targeted dual-mode computed tomography (CT)/magnetic resonance (MR) imaging. The specific binding of tanespimycin to the N-terminal of heat shock protein 90 (HSP90) endows the modified nanoprobes (NPs) with the ability to up-regulate HSP90 tumor cells and to display excellent X-ray attenuation intensity and T MR imaging performance in an orthotopic hepatocellular carcinoma tumor model.

Comparison study between multicontrast atherosclerosis characterization (MATCH) and conventional multicontrast MRI of carotid plaque with histology validation.

Purpose: To compare Multicontrast ATherosclerosis Characterization (MATCH) with conventional multicontrast magnetic resonance imaging (MRI) in the characterization and quantification of carotid plaque components.

Materials And Methods: Fifty-three consecutive patients underwent carotid plaque 3.0T MRI including conventional multicontrast sequences and MATCH, with 13 of them having carotid endarterectomy for histology validation.